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通过循环离子流动性质谱法识别和量化混合物中表皮质的相对度:用德克萨米和贝塔米作为案例研究

Sudam S Mane¹, David V Dearden¹, Kenneth W Lee¹

¹Brigham Young University, Provo, Utah 84602-1030, United States.

Journal of the American Society for Mass Spectrometry

|August 26, 2024

概括

分离像德克萨米他和贝塔米他这样的epimers是具有挑战性的. 循环离子流动性质谱仪通过分析它们的质子二次体,成功地分离和量化了这些体.

科学领域:

分析化学分析化学
药品分析药品分析
分离科学分离科学

背景情况:

体表现出明显的生物活动和药理行为,需要在药物开发过程中精确分离和分析.
类似的epimers的物理化学性质对有效的分离和量化提出了重大挑战.
精确区分epimers对于确保药物的安全性和有效性至关重要.

研究的目的:

证明循环离子流动性质谱法 (cIMS-MS) 的应用,用于分离,识别和量化德克萨米和贝塔米的表皮.
研究德克萨米他和贝塔米他在气相中的质子二元体的形成和行为.
建立一种新的方法,使用cIMS-MS.来准确量化epimers的相对量化.

主要方法:

使用循环离子流动性质谱法 (cIMS-MS) 来分析德克萨米和贝塔米的二元混合物.
分离并分析了epimers的质子化二次体,观察了明显的同聚体和异聚体峰值.
通过注入纯溶液和测量到达时间,确定了特定于epimer的二聚体峰值.

主要成果:

循环IMS-MS成功将德克萨米他和贝塔米他分离为质子二聚体,产生了三个不同的峰值:德克萨米他同聚体,贝塔米他同聚体和它们的异聚体.
异构聚物峰的存在证实了混合物的异构性质,并与单体分析相比提供了更好的分离.
通过分析峰值区域,利用异体聚合物形成率和每个体对异体聚合物信号的平等贡献来计算相对度,实现了量化.

结论:

循环离子流动性质谱学提供了一个有效的平台,用于分离和识别epimers基于他们的质子二次体行为.
这项研究首次通过分析带电二次体来准确地量化epimers的气相相对量化.
开发的方法允许精确识别和量化德克萨米他和贝塔米他,这对于制药开发和质量控制至关重要.

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