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Updated: Jun 15, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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在细菌中使用SSB/CRISPR-Cas9核糖蛋白进行基因编辑

Ran Chai1, Wenying Sun2, Zhixu Xu3

  • 1Henan Engineering Technology Research Center of Green Coating Materials, Yellow River Conservancy Technical Institute, Kaifeng 475004, China; College of Life Sciences, Henan Agricultural University, Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture and Rural Affairs, Zhengzhou 450046, China.

International journal of biological macromolecules
|August 26, 2024
PubMed
概括

这项研究引入了一种新的CRISPR-Cas9核糖蛋白 (RNP) 基因编辑系统,使用DNA单链结合蛋白 (SSB) 进行高效的细菌基因组修改. 通过SSB/CRISPR-Cas9 RNP系统,可以在没有等离子体的细菌中快速简单地进行基因编辑.

关键词:
细菌基因组的编辑通过CRISPR-Cas9进行基因组编辑.没有等离子体的无体.核糖蛋白是一种核糖蛋白.这是一个SSB/CRISPR-Cas9 RNP.

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