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用于ibuprofen生物降解的新细菌菌株:药物去除,转化和潜在的催化基因

Alba Lara-Moreno^1,2, Maria Clara Costa^1,3, Ayleen Vargas-Villagomez³

¹Centre of Marine Sciences (CCMAR/CIMAR LA), University of the Algarve, Gambelas Campus, Faro, Portugal.

Environmental microbiology reports

|August 26, 2024

概括

新发现的细菌可以有效地从废水中去除布洛芬 (IBU),提供可持续的生物修复解决方案. 这些新型菌株表现出高IBU降解率,有助于环境保护.

科学领域:

环境科学环境科学
微生物学微生物学
生物技术是生物技术.

背景情况:

布洛芬 (IBU) 是废水中普遍存在的制药污染物.
在传统处理中,有限的去除会导致环境排放和关注.
使用微生物进行生物修复为IBU缓解提供了一种可持续的方法.

研究的目的:

从各种环境来源分离和识别能够降解布洛芬的新型细菌.
评估这些细菌菌株在从液体介质中去除IBU的效率.
在有前途的细菌分离物中研究IBU生物降解的遗传基础.

主要方法:

从土壤,海洋,矿山和橄厂废水中分离细菌,使用IBU作为唯一的碳来源.
使用16S rRNA基因测序识别细菌菌株.
使用高性能液态染色学 (HPLC) 量化IBU去除.
在全基因组序列的基因分析中,以确定假定的IBU-catabolic基因.

主要成果:

确定了四种细菌菌株 (Klebsiella pneumoniae TIBU2.1,Klebsiella variicola LOIBU1.1,Pseudomonas aeruginosa LOIBU1.2,Mycolicibacterium aubagnense HPB1.1).这些细菌菌株中,最常见的是一种细菌菌.
这些菌株从初始度为5mg/L,在14天内实现了60-100%的IBU去除.
在分析表明,M. aubagnense HPB1.1和K. pneumoniae TIBU2.1具有与IBU代谢途径相关的基因.
在K. pneumoniae TIBU2.1中检测到IBU转化产物,但在M. aubagnense HPB1.1.1.中没有.

结论:

新型细菌菌株,包括K. pneumoniae TIBU2.1和M. aubagnense HPB1.1,显示出对ibuprofen生物修复的显著潜力.
假定代谢基因的存在和相似性与IBU降解效率相关.
对这些细菌菌株的进一步研究可以推进对制药污染物的可持续废水处理策略.

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