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循环的微RNAs作为围产期窒息的生物标志物签名

Yin-Hsuan Lai¹, Yi-Fang Tu², Chia-Huei Chen³

¹Department of Pediatrics, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan; Department of Pediatrics, Camillian Saint Mary's Hospital Luodong, Yilan, Taiwan.

Pediatrics and neonatology

|August 26, 2024

概括

微RNA-486-5p显示为新生儿周产期窒息的生物标志物具有前途. 这种微RNA的较低水平与窒息严重程度和不良结果相关.

科学领域:

新生儿医学新生儿医学
分子生物学分子生物学
生物标志物发现发现

背景情况:

围产期窒息是新生儿发病率和死亡率的重要原因之一.
确定围产期窒息的可靠生物标志物对于及时诊断和干预至关重要.
微RNA (miRNA) 正在成为各种疾病的潜在诊断和预后指标.

研究的目的:

研究特定的微RNAs (miRNAs) 作为围产期窒息的生物标志物的潜力.
评估miRNA表达水平与磁共振成像 (MRI) 显示的大脑损伤严重程度之间的相关性.

主要方法:

预计将招收26名满期新生儿 (10名患有围产窒息,16名对照).
在分娩后0-6小时和7天收集血样本.
下一代测序和定量逆转录实时聚合酶链反应 (RT-PCR) 用于miRNA表达分析.
修改了对脑病变的萨尔纳特分期和对脑损伤评估的MRI评分.

主要成果:

与对照组相比,在窒息的新生儿中0-6小时观察到miR-486-5p的显著较低表达 (p=0.005).
miR-486-5p在区分围产期窒息症 (AUC=0.831) 和确定治疗性低温症候选者的诊断准确度很好 (AUC=0.782).
降低7天的miR-486-5p表达与窒息新生儿的不良结果相关.

结论:

在新生儿中,MiR-486-5p可能作为围产期窒息的有价值生物标志物.
需要进一步的研究,以充分阐明miR-486-5p在围产期窒息中的作用及其预后影响.

关键词:

生物标志物生物标志物低毒性-缺血性脑病变是一种脑病变.周围产生的窒息症在 miR-486-5p 中使用.这是一个微型RNA.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...