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关于优化Phyllopora lonicerae菌体液体发酵介质和抗瘤活动的研究

Min Liu¹, Lu Liu¹, Guoli Zhang¹

¹Shandong Province Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, P.R. China.

Journal of microbiology and biotechnology

|August 26, 2024

概括

菲洛波里亚 (Phylloporia lonicerae) 菌,优化了发酵,显示出显著的抗瘤潜力. 它的石油提取物有效地诱导癌细胞的亡,为功能性食物的发展铺平了道路.

科学领域:

菌类学菌类学是指菌类学.
自然产品化学自然产品化学
癌症研究癌症研究

背景情况:

菲洛波利亚 (Phylloporia lonicerae) 是一种寄生真菌,具有潜在的药用价值.
有限的野生资源和不清楚的抗瘤机制阻碍了它的发展.
优化发酵和提取对于利用其生物活性化合物至关重要.

研究的目的:

为了优化发酵媒介用于Phylloporia lonicerae菌生产.
为了研究P. lonicerae菌提取物的抗瘤活性.
为潜在的功能性食品应用确定和描述抗癌成分.

主要方法:

使用包括Lonicera japonica在内的各种成分来优化发酵媒介.
使用酒精和水提取细胞内成分.
酒精提取物的分级提取和对抗A549和Eca-109细胞系的抗增殖活性的评估.
流细胞计分析以确定作用机制 (亡诱导).

主要成果:

优化的介质显著增加了生物质 (142%) 和减少了种植时间 (64%).
细胞内酒精提取物显示出比水提取物更高的细胞毒性.
酒精提取物的石油相表现出优异的抗癌活性 (IC50 = 113.3 μg/ml),并在Eca-109细胞中诱导了亡.

结论:

优化的发酵和提取协议提高了P. lonicerae菌的生产和生物活性化合物的产量.
石油提取物成分具有显著的抗癌性质,主要是通过诱导亡.
作为功能性食品中新型抗癌剂的来源,P. lonicerae具有前景.

关键词:

菲洛波里亚 (Phylloporia lonicerae) 是一个孤独的植物.它具有抗癌活性,具有抗癌活性.细胞亡是细胞的亡.液体发酵液体发酵是什么响应表面方法的方法.

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