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在Caudoviricetes细菌菌体中进行DNA聚合酶交换

Natalya Yutin¹, Igor Tolstoy¹, Pascal Mutz¹

¹National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA.

Virology journal

|August 26, 2024

概括

DNA聚合酶 (DNAP) 交换在尾巴菌体中经常发生,导致与无关DNAPs密切相关的菌体. 这种进化可能有助于菌体逃避宿主防御和复制冲突.

科学领域:

病毒学病毒学
分子生物学分子生物学
进化生物学进化生物学

背景情况:

在Duplodnaviria领域的病毒具有双链DNA基因组和保存的基因模块,但在DNA复制蛋白中存在差异.
DNA聚合酶 (DNAPs) 对于DNA复制至关重要,并被分为四个无关家族 (A-D).
之前的研究表明,Crassvirales可以编码来自不同家族的DNAPs的密切相关的病毒.

研究的目的:

为了研究DNA聚合酶 (DNAP) 基因家族在尾巴菌体中的进化动态.
在Caudoviricetes类中识别DNAP基因交换的实例和模式.

主要方法:

构建了尾状细菌的综合进化树,包括具有不同DNAP家族的细菌.
经过验证的DNAP交换使用大终结酶子单元和毛虫基因组对齐的受约束树分析.
使用AlphaFold2.2. 预测了新型DNAPs的结构.

主要成果:

确定了四个额外的尾巴菌体群,在A,B和C家族之间进行了多次DNAP基因交换.
观察到的DNAP交换发生在"现场",而不会改变周围的基因组织.
在一些菌体基因组中发现了两个新的,高度分离的家族A DNAPs组.

结论:

不同家族的DNAP基因替代在尾巴菌体进化过程中多次独立发生.
菌体DNAP交换可能是由于需要逃避宿主抗菌体机制或避免复制体不兼容而导致的.
这种进化过程可以导致密切相关的菌体编码无关的DNAPs.

关键词:

细菌抗病毒防御的防御方法这种DNA聚合酶是DNA聚合酶.病毒的演变病毒的演变横向的基因转移是可以实现的.

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