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在使用多重PCR测试的儿童中,PCR阳性的呼吸道病毒类型的持续时间

Taito Kitano¹, Daisuke Kitagawa², Masayuki Murata¹

¹Department of Pediatrics, Nara Prefecture General Medical Center, Nara, Japan.

Journal of medical virology

|August 27, 2024

概括

持久阳性聚合酶连锁反应 (PCR) 测试在患有呼吸道病毒的儿童中很常见. 这项研究发现PCR阳性对不同病毒的持续时间不同,影响日常生活和感染控制措施.

科学领域:

儿童传染病儿童传染病
临床病毒学临床病毒学
分子诊断学分子诊断

背景情况:

长期呈阳性聚合酶链反应 (PCR) 结果可能导致扩大限制和感染控制措施.
关于单个儿科队列内多个呼吸道病原体的PCR阳性持续时间的数据有限.

研究的目的:

评估和比较PCR阳性对儿童和青少年各种呼吸道病毒的持续时间.
确定影响该人群持续PCR阳性的因素.

主要方法:

在90天内进行至少两次PCR测试的症状儿童和青少年的回顾性分析.
使用多重PCR呼吸板进行测试.
对多种病毒持续PCR阳性的评估率和可能性.

主要成果:

持续的PCR阳性是常见的,随着时间的推移而下降 (60.6%在1-15天到21.7%在76-90天).
鼻病毒/肠病毒和腺病毒显示持续阳性的可能性增加.
与类型流感病毒相比,流感和季节性冠状病毒的持续阳性可能性降低.

结论:

持久的PCR阳性是多种呼吸道病毒在症状儿童中经常发生的情况.
了解PCR阳性的差异持续时间对于管理感染控制和患者护理至关重要.

关键词:

根据COVID-19的情况,在SARS-CoV-2中.腺病毒的病毒.流感流感流感流感流感复合聚合酶连锁反应的多重反应呼吸道同胞性病毒感染呼吸道感染呼吸道感染

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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...