在Mycobacterium smegmatis中通过抑制呼吸电子运输链来减少核糖体蛋白基因的表达
Na-Kyeong Kim1, Jong-Eun Baek1, Ye-Jin Lee1
1Department of Integrated Biological Science, Pusan National University, Busan, Republic of Korea.
Frontiers in microbiology
|August 27, 2024
概括
在Mycobacterium smegmatis中,抑制呼吸电子运输链 (ETC) 通过依赖Rel的严格反应 (SR) 降低了核糖体基因表达. 恢复Rel功能可以防止这种下降,突出显示SR.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 细菌生理学 细菌生理学
背景情况:
- 呼吸电子运输链 (ETC) 对于细菌的能量生产至关重要.
- 终端氧化酶,如a3细胞染色体c氧化酶,是ETC的关键组成部分.
- 严格反应 (SR) 是细菌的全球调节机制.
研究的目的:
- 为了研究呼吸电子运输链 (ETC) 抑制对Mycobacterium smegmatis. ribosomal基因表达的影响.
- 阐明严格反应 (SR) 和Rel蛋白在这个过程中的作用.
- 确定涉及ETC抑制诱导的基因表达变化的调节途径.
主要方法:
- 构建和分析Mycobacterium smegmatis的Daa3突变体.
- 核糖体蛋白基因的基因表达分析.
- 研究rel基因及其产物 (p) pGpp合成酶的作用.
- 暴露于各种呼吸抑制条件 (KCN,贝达基林,缺氧,bcc1复杂突变).
- 对MprBA-SigE-SigB调控途径的分析.
主要成果:
- 在Mycobacterium smegmatis中抑制aa3终端氧化酶会导致核糖体蛋白基因的表达减少和核糖体含量减少.
- 删除rel基因恢复了Δaa3突变体中的核糖体蛋白基因表达,表明了依赖Rel的严格反应 (SR).
- 过度表达Rel降低了核糖体基因表达,而其在Δaa3突变体中的表达增加支持了依赖Rel的SR诱导.
- 通过KCN,贝达基林,缺氧或bcc1复杂突变抑制呼吸也会降低核糖体基因表达.
- MprBA-SigE-SigB通路参与了 Δaa3 突变体中增加的 rel 表达和减少的核糖体基因表达.
结论:
- 在Mycobacterium smegmatis中抑制呼吸道ETC引发了依赖Rel的严格反应 (SR).
- 这种SR导致核糖体蛋白基因表达的下调.
- MprBA-SigE-SigB通路在呼吸应激下调解这些影响方面发挥着作用.
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