相关实验视频

Updated: Jun 15, 2025

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Published on: November 5, 2020

关于Ichthyscopus pollicaris的整个基因组数据集

Tianxiang Gao¹, Wenyu Li¹, Yinquan Qu¹

¹Fishery College, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.

|August 27, 2024

概括

这项研究介绍了第一个 Ichthyscopus pollicaris 的全基因组序列,这是一种Uranoscopidae物种. 生成的基因组数据将有助于理解鱼类的进化和分类学.

科学领域:

海洋生物学海洋生物学
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

乌拉诺斯科皮类动物物种的分类是复杂的,也是有争议的.
2019年确定了Uranoscopidae家族的一种成员Ichthyscopus pollicaris.

研究的目的:

为了生成第一个Ichthyscopus pollicaris的全基因组序列.
分析基因组结构并注释关键的遗传元素.
使用单复制的正统基因重建家族遗传关系.

主要方法:

使用PacBio和Illumina平台进行全基因组测序.
新的基因组组装和校正.
重复序列,基因,miRNAs,tRNAs,rRNAs和snRNAs的注释. 这是一个很好的方法.
与其他11个物种进行了家族遗传学分析.

主要成果:

一个527.25 Mb的Ichthyscopus pollicaris基因组被组装在一起,N50为11.25 Mb.
标注的元素包括170.41 Mb的重复序列,21,263个基因,784个miRNA,2,225个tRNA,3004个rRNA和1422个snRNA.
通过使用3,168个单复制的正统基因进行了家族遗传学分析.

结论:

该基因组草案为Ichthyscopus pollicaris的研究提供了宝贵的资源.
这些基因组数据将有助于解决Uranoscopidae分类学和进化研究.
这项研究为未来对这种鱼类家族的基因组研究奠定了基础.

关键词:

基因组基因组基因组基因组基因组这种鱼类叫做Ichthyscopus pollicarisaris.遗传学关系遗传学关系第三代测序是第三代测序.

更多相关视频

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

Published on: October 5, 2017

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Published on: September 20, 2016

相关概念视频

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...