一种蓝藻细菌的西格玛因子F通过细胞内和细胞间通路控制生物膜促进基因
Shiran Suban1, Sapir Yemini1, Anna Shor1
1The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, Israel.
Biofilm
|August 27, 2024
概括
RNA聚合酶西格玛因子SigF1通过控制柱状聚合物和细胞外抑制剂分泌来调节蓝菌生物膜的形成. 这一发现揭示了蓝藻细菌细胞间通信中的一个关键机制.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 菌形成具有工业意义的摄影性生物膜.
- 之前在 *Synechococcus elongatus* PCC 7942.4 中观察到一种涉及细胞外抑制剂的生物膜抑制机制.
- 这种机制表明在蓝藻细菌中细胞间通信的作用.
研究的目的:
- 研究RNA聚合酶西格玛因子SigF1和SigF2在*Synechococcus elongatus*PCC 7942.2.生物膜抑制机制中的作用.
- 阐明控制蓝藻细菌生物膜形成和细胞间通信的调节途径.
主要方法:
- 在 *Synechococcus elongatus* 中 *sigF1* 和 *sigF2* 的遗传失活化 PCC 7942.
- 转录组分析以确定由SigF1和SigF2控制的基因调节子.
- 在条件介质中分析柱状组合和细胞外抑制剂的存在.
主要成果:
- SigF1,但不是SigF2,对于生物膜抑制机制至关重要.
- 失去了SigF1导致减少了pilus亚单元基因的转录,防止了pilus组装和抑制剂分泌.
- SigF1的不激活可以提高基因基因的基因组件和分泌系统的调节,这表明生物膜促进因子的减压.
结论:
- SigF1是蓝色细菌细胞间通信的关键调节者,控制生物膜的形成.
- 柱状组合复合体参与生物膜抑制剂的分泌.
- SigF1对生物膜发育具有细胞外 (通过抑制剂) 和细胞内调节作用.
相关概念视频
Bacterial Signaling
31.8K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
31.8K
Cytoskeletal Proteins in Bacteria
3.3K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
3.3K
Bacterial RNA Polymerase
29.4K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
29.4K
Combinatorial Gene Control
8.3K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.3K
Prokaryotic Transcriptional Activators and Repressors
20.9K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
20.9K


