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Updated: Jun 15, 2025

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

Published on: June 8, 2012

测试纳米粒子反应剂用于成像质量细胞计.

Mahtab Abtahi¹, Ladan Gheiratmand², Anuroopa Dinesh³

¹Department of Chemistry, University of Toronto, Toronto, Ontario M5S 3H6, Canada.

Biomacromolecules

|August 27, 2024

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此摘要是机器生成的。

新的纳米粒子报告器增强了成像质量细胞计 (IMC) 灵敏度,用于检测罕见的生物标志物. 使用聚硫贝甲基酸) - 尼里德罗酸盐 (PSBMA-Ner) 的涂层有效地减少了组织样本中的非特异性结合.

科学领域:

生物医学工程生物医学工程
分析化学分析化学
材料科学材料科学材料科学

背景情况:

质量细胞计 (MC) 是一种强大的单细胞分析工具.
低丰度生物标志物的检测仍然是MC的一个挑战.
纳米粒子 (NP) 试剂可以通过携带多个重金属同位素来提高MC灵敏度.

研究的目的:

开发用于成像质量细胞计 (IMC) 的新型纳米粒子 (NP) 记者.
为了提高MC分析的灵敏度和特异性.
研究NP表面修饰对非特异性结合 (NSB) 的影响.

主要方法:

合成的 NaYF4:Yb3+/Er3+ NPs. 这些是
使用两步联体交换与甲基-PEG2K-neridronate (PEG-Ner) 和/或聚硫甲基酸-neridronate (PSBMA-Ner) 的功能化NP表面.
在PBS缓冲器中评估了体稳定性,并对桃体和乳腺癌组织进行了IMC测量.

主要成果:

两种PEG-Ner和PSBMA-Ner涂层都提供了长期的体稳定性.
PSBMA-Ner或1:1 PSBMA-Ner + PEG-Ner混合物有效地抑制了桃体组织中的NSB在2 × 10^10NP/mL.

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在乳腺癌组织中,在NP度超过2×10^10NP/mL时,观察到NSB的增加.

结论:

使用PSBMA-Ner或混合PEG-Ner/PSBMA-Ner涂层对NP的表面修改可以显著减少IMC中的NSB.
这些发现为开发NP合物为更敏感和更具体的MC分析铺平了道路.
异生物功能的PEG可用于创建先进的MC应用的NP-生物亲和剂结合物.