使用H定量核磁共振光谱学对Mitragyna speciosa选定的类化合物的定量分析
Suleiman Abubakar Garba1,2, Khozirah Shaari1, Mohd Rashidi Abdul Manap3
1Natural Medicines and Products Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Malaysia.
Magnetic resonance in chemistry : MRC
|August 27, 2024
概括
定量核磁共振 (qNMR) 谱学提供了一种快速而精确的方法来检测Mitragyna speciosa中的精神活性化物. 与其他植物部位相比,红色的叶子含有显著更高的类化合物.
科学领域:
- 分析化学 分析化学
- 药理学是指药理学,即药理学是指药理学.
- 频谱学是一种光谱学.
背景情况:
- 亚洲原产的一种植物Mitragyna speciosa含有诸如mitragynine之类的精神活性类化合物,这些类化合物以其镇静性和高兴效应而闻名.
- 滥用M. speciosa导致了成,突出了需要有效的分析方法来检测其成分.
- 传统的基于色谱的方法耗时且昂贵,因此需要采用替代方法.
研究的目的:
- 开发一种快速,精确和非破坏性的定量核磁共振 (qNMR) 光谱方法,用于量化M. speciosa.中主要的精神活性类化合物.
- 为了比较M. speciosa的不同部分的化物含量,包括叶子 (红色和绿色),茎皮和果实.
主要方法:
- 定量核磁共振 (qNMR) 光谱法用于化物量化.
- 使用相对于内部标准 (1,4-dinitrobenzene) 的 -OCH3 组的积分值来量化mitragynine,specioliatine 和 speciogynine.
- 方法验证包括对精度,可重复性,特异性,灵敏性,线性,LOD和LOQ的评估.
主要成果:
- 开发的qNMR方法显示出高精度和可重复性,相对标准偏差 (RSD) 为2%.
- 该方法表现出极好的特异性,灵敏度,广泛的线性范围 (R2 = 0.999) 和有利的LOD/LOQ值.
- 与绿色的叶子,茎皮或果实相比,红色的M. speciosa叶子含有显著更高的米特拉基宁 (32.34 mg/g),特种酸 (16.84 mg/g) 和特种酸 (7.69 mg/g).
结论:
- 定量核磁共振 (qNMR) 谱学为分析M. speciosa类的传统方法提供了可靠,快速和精确的替代方案.
- 这些发现表明,红色的M. speciosa叶片是主要精神活性类化合物的更丰富来源.
- 这种方法有助于精确量化和比较不同M. speciosa样本中的化物概况.
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