效应因子SJP3通过维持朱中的SHORT VEGETATIVE PHASE 3表达来干扰的发育
Mingsheng Deng1,2, Fuli Ma1, Liping Zhai1
1Anhui Province Key Laboratory of Horticultural Crop Quality Biology, College of Horticulture, Anhui Agricultural University, 130 West Changjiang Road, Hefei City 230036, Anhui Province, People's Republic of China.
Plant physiology
|August 27, 2024
概括
朱花的女巫们是一个魔女.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 朱魔女的扫 (JWB) 是一种植物等离子体疾病,会损害朱作物.
- 植物质体导致花逆转,导致花和植物生长.
- 众所周知,分泌的JWB蛋白3 (SJP3) 能够诱导植物生长.
研究的目的:
- 为了阐明SJP3介导的玉树中杆逆转的分子机制.
- 确定与SJP3相互作用的蛋白质及其在疾病发展中的作用.
主要方法:
- 通过使用转基因calli.研究了SJP3和ZjSVP3之间的相互作用.
- 在健康和受感染的朱树组织中分析了基因表达模式.
- 在 *Nicotiana benthamiana* 进行异质表达,以研究 ZjSVP3 功能.
- 检查了ZjSVP3对ZjZFP8和ZjSHP1.1目标基因的促进体结合.
主要成果:
- SJP3与MADS盒蛋白ZjSVP3相互作用并使其稳定,防止其降解.
- 在JWB感染的花朵中,ZjSVP3表达被构成性地维持.
- ZjSVP3诱导的杆异常的异质表达.
- ZjSVP3直接调节了ZjZFP8和ZjSHP1的表达,影响了的发育.
结论:
- SJP3效应器稳定了ZjSVP3,维持了它的表达,并扰乱了正常的茎发育.
- 这种相互作用提供了JWB植物质素诱导的朱树回归的分子机制.
- 这些发现提供了关于多年木质物种中植物等离子体相互作用的见解.
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