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拉菲诺环素是一种新型的塑体编码的圆形细菌素,由Lactococcus raffinolactis生产,具有针对许多格拉姆阳性食品病原体的广泛活性

Felipe Miceli de Farias¹, Paula M O'Connor², Colin Buttimer¹

¹APC Microbiome Ireland, University College Cork, Cork, Ireland.

Applied and environmental microbiology

|August 27, 2024

概括

一种新型的循环细菌素,拉菲诺环素,在生牛奶细菌Lactococcus raffinolactis中发现. 这种抗微生物对病原体具有广泛的活性,并具有作为天然食品生物防腐剂的潜力.

科学领域:

微生物学微生物学
生物化学生物化学
食品科学食品科学食品科学

背景情况:

细菌素是抗微生物,在食品工业中具有生物技术潜力.
乳球菌 (Lactococcus raffinolactis) 是一种乳酸细菌,在生牛奶中发现.
新型抗菌剂正在寻求用于食品保存和病原体控制.

研究的目的:

发现和描述一种来自Lactococcus raffinolactis的新型细菌素.
确定新型细菌素的抑制光谱和稳定性.
分析细菌素生产的遗传基础.

主要方法:

从生牛奶中分离和表征乳球菌拉菲诺菌APC 3967.
确定细菌素的分子质量,氨基酸序列,以及已知细菌素的身份.
对各种细菌菌株的抑制光谱的检测.
对细菌素对酶处理的稳定性的分析.
细菌素基因集群的全基因组测序和遗传分析.

主要成果:

发现了拉菲诺环素,这是由L. raffinolactis生产的61氨基酸圆形细菌素.
拉菲诺环素表现出广泛的抗菌活性,包括对抗食物传播的病原体,如李斯特菌和葡萄球菌.
细菌素在很大程度上与细胞相关,对素的敏感性较低.
拉菲诺环素的基因集群涉及10个基因,包括一个ABC转运器和一个转录调节器.
这是首次报告来自L. raffinolactis物种的细菌素.

结论:

拉菲诺环素是一种新型的,用等离子体编码的圆形细菌素,具有显著的抗菌特性.
它的广泛的抑制光谱和稳定性表明它有可能成为食品工业中的天然生物防腐剂.
拉菲诺环素的遗传元素可以用于将抗菌能力转移到其他乳酸细菌中,用于工业应用.

关键词:

乳球球菌拉菲诺拉克提斯.抗微生物活性的抗微生物活性.抗微生物是一种抗微生物.这是一种圆形的细菌.拉菲诺环素是一种

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