从脱污染的表面快速和灵敏地确定剩余子传染性
Sara M Simmons1, Vivianne L Payne2, Jay G Hrdlicka1
1Department of Medical Microbiology and Immunology, School of Medicine, Creighton University, Omaha, Nebraska, USA.
mSphere
|August 27, 2024
概括
使用实时震动诱导转换 (RT-QuIC) 进行表面擦拭,可以在消毒后检测剩余的子. 不同于水或乙醇,二酸盐可以有效地去污染表面,确保实验室和诊所的安全.
科学领域:
- 神经科学是一个神经科学.
- 传染性疾病 传染性疾病
- 生物化学 生化学
背景情况:
- 子疾病是致命的,由错误折叠的子蛋白 (PrPSc) 引起的传染性神经退行性疾病.
- 子可以污染实验室和临床环境中的表面,造成意外传播的风险.
- 有效的子去污方法对于预防疾病传播至关重要.
研究的目的:
- 评估表面擦拭与实时震动诱导转换 (RT-QuIC) 结合的有效性,以检测消毒后的剩余子.
- 为了比较不同净化剂 (水,70%乙醇,二) 在消除与表面相关的子方面的有效性.
- 为了验证RT-QuIC测定与动物生物测定对子检测的有效性.
主要方法:
- 在受污染的材料 (实验室和临床表面,不钢电线) 上进行表面擦拭.
- 使用水,70%乙醇或酸盐消毒受污染的表面.
- 使用实时震动诱导转换 (RT-QuIC) 检测片提取物中的剩余子.
- 将RT-QuIC结果与动物生物试验进行比较.
主要成果:
- 水和70%乙醇处理显示出强大的检测与表面相关的子.
- 盐酸过酸盐处理导致无法检测到与表面相关的子,这表明有效的去污.
- 对不钢电线和片提取物的RT-QuIC分析显示与动物生物测试一致.
- 识别了可能影响RT-QuIC测定性能的干扰物质.
结论:
- 表面擦拭与RT-QuIC相结合,是一种可行的方法,用于评估各种表面上离子去污染的有效性.
- 低酸是一种有效的消毒剂,可以防止与表面相关的.
- RT-QuIC 作为用于子检测的动物生物测试的可靠和快速替代品,增强了研究和医疗机构的安全措施.
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