现场可部署的色度测量阵列用于现场诊断尿路感染和识别致病原体
Xianming Li1,2, Ting Zheng1, Yuling Xiao3
1Analytical & Testing Center, Sichuan University, Chengdu 610064, Sichuan, China.
Analytical chemistry
|August 27, 2024
概括
一个新的便携色度阵列 (PoCA) 通过在两个小时内识别12种常见病原体,使尿路感染 (UTI) 的快速诊断成为可能. 这一进步有助于适当的治疗,并打击在资源有限的环境中滥用抗生素.
科学领域:
- 微生物学 微生物学
- 临床诊断 临床诊断 临床诊断
- 照顾点检测检测 照顾点检测
背景情况:
- 尿路感染 (UTI) 很常见,由于诊断局限性导致不适当的治疗,特别是在资源有限的地区.
- 目前的诊断方法往往缺乏及时干预所需的速度,可移植性和质量,导致全球滥用抗生素.
- 快速而准确的病原体鉴定对于有效的尿路感染管理和抗菌药物管理至关重要.
研究的目的:
- 开发和验证一个便携式色谱阵列 (PoCA),用于快速,高通量检测尿道感染病原体.
- 调整以前开发的基于DNA的色度测试方法,用于用迷你LED辐射照射的纸盘.
- 与标准尿液培养方法相比,评估PoCA平台的诊断准确性.
主要方法:
- 设计了一种定制的便携式色谱阵列 (PoCA),将迷你LED照射与纸盘集成,用于读取聚合酶链反应 (PCR) 片.
- 该PoCA平台与用于PCR放大的便携式热循环器相结合.
- 使用PoCA系统分析了200个临床尿样,以确定12种流行性尿路感染病原体.
主要成果:
- 在不到2小时的时间里,PoCA平台从96个PCR测试中成功诊断和识别了12个尿路感染病原体.
- 发现PoCA的病原体分析精度优于标准尿液培养方法.
- 下一代元基因组测序证实了PoCA系统的高精度.
结论:
- 便携式色谱阵列 (PoCA) 为尿路感染诊断和病原体识别提供了快速,准确和高通量解决方案.
- PoCA 平台在初级保健环境中具有很大的使用潜力,特别是在资源有限的地区.
- 这项技术可以改善尿路感染的治疗,并通过及时和精确的诊断,有助于减少滥用抗生素.
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