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Updated: Jun 15, 2025

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

同义代码子置换通过调节反意义RNA生产来调节一个不同的上游基因的转录和翻译

Anabel Rodriguez¹, Jacob D Diehl¹, Gabriel S Wright²

¹Department of Chemistry & Biochemistry, University of Notre Dame, Notre Dame, IN 46556.

Proceedings of the National Academy of Sciences of the United States of America

|August 27, 2024

概括

一个基因中的同义编码子变化可以通过创建反意义RNA来意外地促进邻近基因的表达. 这表明细菌可能会选择使用子来避免破坏附近的基因表达.

科学领域:

分子生物学分子生物学
遗传学遗传学是一个
微生物学微生物学

背景情况:

同义代码子在历史上被认为是功能等价的.
新出现的证据表明同义替代影响基因表达和蛋白质平衡.
之前的研究主要检查了单个基因内的影响.

研究的目的:

为了调查一个基因中的同义代码子替代是否会影响一个不同的上游基因的表达.
探索这种效应发生的机制.
了解对细菌中的编码子使用选择的影响.

主要方法:

在Escherichia coli等离子体上的氨基醇乙转移酶 (猫) 基因中工程化同名代码子替代.
测量了上游四环素耐药性 (tetR) 基因的表达水平.
分析RNA转录,包括反意义RNA (asRNA) 和蛋白质积累.

主要成果:

猫基因的同义重编码在九种变异中的四种变异中显著增加了tetR基因表达.
这种增长是由从猫体内的起点转录的反感性RNA调解的.
反感性RNA转录绕过了原生TetR抑制,TetR蛋白水平与asRNA相关,而不是总的TetRRNA.

结论:

同义代码子的使用可以影响邻近基因的转录和翻译.
细菌的编码子选择可以平衡氨基酸的保存,避免干扰相关基因表达的元素.
这些发现对于理解密集 prokaryotic 基因组中的基因调节和合成生物学应用具有重要意义.

关键词:

这是一种反义的RNA.基因表达的基因表达方式相互重叠的不同的促进者.蛋白质的平衡是蛋白质的平衡.同义词的代码子替代.

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