抗生素耐药性基因从土壤转移到田中的大米
Min Xu1, Fang Wang2, Robert D Stedtfeld3
1CAS State Key Laboratory of Soil & Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 211135, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Environment international
|August 27, 2024
概括
猪肥料在米土壤和米根中显著增加了抗生素耐药性基因 (ARG),但米粒中的ARG不受施肥的影响. 需要进一步的研究来确定米粒中的ARG来源.
科学领域:
- 环境微生物学环境微生物学
- 农业科学 农业科学
- 食品安全 食品安全
背景情况:
- 抗生素耐药性基因 (ARG) 是一个日益严重的全球问题.
- 了解环境矩阵之间的ARG传输至关重要.
- 关于ARG从土壤转移到作物上的数据有限.
研究的目的:
- 在不同的肥料条件下,研究ARG从土壤转移到大米中的情况.
- 量化不同生长阶段在土壤和大米中的ARG丰度和多样性.
- 评估猪肥料对ARG概况的影响.
主要方法:
- 实地研究使用三个施肥方法:化学肥料,猪肥料和没有肥料.
- 在多个时间点 (0,7,30,79天) 采集土壤和大米样本.
- 高通量定量PCR (qPCR) 使用144个原始剂量化ARG.
主要成果:
- 猪堆肥显著增加了在田土壤中的ARG丰度和基因拷贝数量.
- 在米苗中检测到ARG,在移植后的丰度下降.
- 在土壤和大米根中,ARG的频率在收获时使用猪肥料时增加.
- 在不同处理的米粒中,没有发现ARG丰度或多样性的显著差异.
结论:
- 施肥类型,特别是猪肥料,会影响田土壤和大米根的ARG动态.
- 灌水可能会导致土壤中的ARG.
- 在米粒中ARG的来源需要进一步调查.
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