斯库特拉林可以通过引发线粒细胞衰变来改善OGD/R侵袭HT22细胞中的线粒体功能障碍和亡
Lu Yang1, Xianfeng Liu2, Siyin Chen2
1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, China; State Key Laboratory of Southwestern Chinese Medicine Resources, Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; Meishan Hospital of Chengdu University of Traditional Chinese Medicine, Meishan 620010, China.
斯库特拉林通过改善线粒体功能和减少细胞死亡来保护大脑细胞免受损伤. 这种神经保护作用是通过增强细胞循环,细胞循环过程的线粒细胞吸收来实现的.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 斯库特拉林 (Scu) 是一种来自Erigeron breviscapus的黄类物质,具有神经保护的潜力.
- 它在线粒体保护大脑缺血中的作用需要进一步阐明.
研究的目的:
- 研究Scutellarin在HT22细胞中对缺氧-葡萄糖和再注射 (OGD/R) 损伤的保护作用背后的机制.
- 为了确定Scutellarin的神经保护是否涉及线粒体增强和线粒体刺激.
主要方法:
- 为了诱导损伤,HT22细胞接受了OGD/R.
- 施用了斯库特拉林治疗,并评估了其对活性氧物种 (ROS),亡标志物 (TUNEL,Caspase-3,Cytochrome c),线粒体功能 (MMP,OCR,ATP,Na+/K+-ATPase) 和线粒体的标志物 (LC3,Beclin1,PINK1,Parkin,p62) 的影响.
- 用电子显微镜评估线粒体功能障碍.
- 通过使用Mdivi-1,一种线粒抑制剂,证实了线粒的作用.
主要成果:
- 斯库特拉林显著降低了细胞和线粒体ROS,氧化应激和亡.
- 它保留了线粒体形态和功能,由改善的线粒体膜潜力 (MMP),氧消耗率 (OCR),ATP生产和Na+/K+-ATPase活性所证明.
- 斯库特拉林通过上调关键蛋白质 (LC3,Beclin1,PINK1,Parkin) 和增强p62降解来促进线粒.
- 使用Mdivi-1抑制线粒代谢,逆转了Scutellarin对细胞活力,线粒体功能和亡的保护作用.
结论:
- 斯库特拉林通过增强线粒体功能和抑制亡来减轻OGD/R诱导的HT22细胞损伤.
- 刺激线粒是调解斯库特拉林神经保护作用的关键机制.
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