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在临近分娩的母马中评估循环中的miRNAs

Mio Kikuchi¹, Harutaka Murase², Kenichi Urata²

¹Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan; Graduate School of Veterinary Sciences, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.

Domestic animal endocrinology

|August 27, 2024

概括

研究人员在马匹中确定了特定的循环microRNAs (miRNAs),这些microRNAs在分娩前变化. 这些新型和已知的miRNAs显示出作为生物标志物的潜力,用于预测生育日期.

科学领域:

兽医科学兽医科学兽医科学
分子生物学分子生物学
生殖生物学生殖生物学

背景情况:

循环微RNAs (miRNAs) 是体液中的稳定生物标志物.
虽然在哺乳动物中已知怀孕miRNAs,但母马分娩miRNA研究很少.
预测分娩日期对于马类管理至关重要.

研究的目的:

确定马匹中与分娩相关的循环miRNAs.
研究它们作为预测分娩日期的生物标志物的潜力.
建立马匹分娩miRNAs的基础知识.

主要方法:

在分娩前30,5和0天从纯血统马收集血.
进行了小RNA测序 (小RNA-seq) 和反转录定量PCR (RT-qPCR).
使用酶相关免疫吸收试验测量血孕激素度.

主要成果:

小RNA-seq确定了18个受分娩近距离影响的miRNA.
两个新的和三个已知的miRNAs (miR-361-3p,miR-483,miR-99a) 在分娩前5至0天显著变化.
血孕激素水平在分娩前5-3天增加,并在分娩日下降.

结论:

这项研究为与马匹分娩相关的循环miRNAs提供了新的见解.
确定了特定的miRNAs (miR-361-3p,miR-483,miR-99a) 作为预测分娩的潜在生物标志物.
贡献了必要的数据,用于对马的幼的非侵入性预测.

关键词:

生物标志物生物标志物马马的马,就是马马.分割的方法分割的方法怀孕怀孕怀孕怀孕微RNAs 是一个微型RNA.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...