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对河流生物膜的 prokaryotic 社区的影响:对它们的殖民动力学,结构和功能的影响
Sarah Gourgues1, Marisol Goñi-Urriza1, Mathieu Milhe-Poutingon1
1Universite de Pau et des Pays de l'Adour, E2S-UPPA, CNRS, IPREM, Pau, France.
The Science of the total environment
|August 27, 2024
概括
(Co) 污染显著改变了河流生物膜的 prokaryotic 社区,从菌转移到 Bacteroidetes 的统治地位. 生物膜在去除后没有恢复,表明它对水生生态系统的持久影响.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 生态毒理学 生态毒理学
背景情况:
- (Co) 对于低碳技术至关重要,但其对环境的影响尚不清楚.
- 河流生物膜是对环境变化敏感的重要水生生态系统.
- 生物膜中的 Prokaryotic 社区可以作为水污染的生物指标.
研究的目的:
- 调查污染对河流生物膜 prokaryotic 社区的影响.
- 评估这些社区作为污染生物指标的潜力.
- 评估生物膜社区在停止暴露后的弹性.
主要方法:
- 生物膜是在人工流中培养的,其度不同 (0.1,0.5,1μM).
- 在28天内使用定量PCR和DNA元编码来分析 Prokaryotic 的丰富性和多样性.
- 通过在额外的35天内去除来测试社区的弹性.
主要成果:
- 的度为0.5和1μM从一开始就显著改变了 prokaryotic 社区的组成.
- 在暴露于的生物膜中,Bacteroidetes取代了菌和浮游生物,成为占主导地位的类.
- 非光合作用 prokaryotes 在高应力下使用了替代碳固定路径.
- 在去除后,没有观察到生物膜社区的结构恢复.
结论:
- 河流生物膜中的 Prokaryotic 社区结构是污染的敏感指标.
- 分析 prokaryotic 社区的分子方法可以有效地评估由于的水质变化.
- 污染导致水生微生物生态系统的持续变化.
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