在具有高皮质醇背景的初级牛子宫内膜层细胞中引起了增强的抗炎作用
Luying Cui1,2,3, Min Zhang1,2,3, Fangling Zheng1,2,3
1College of Veterinary Medicine, Yangzhou University, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, Jiangsu, PR China.
BMC veterinary research
|August 27, 2024
概括
补充剂减少了牛子宫内膜细胞的炎症,其抗炎作用由高水平的皮质醇增强. 这表明在产后子宫健康方面具有潜在的治疗作用.
科学领域:
- 兽医免疫学 兽医免疫学
- 细胞生物学 细胞生物学
- 生殖医学 生殖医学
背景情况:
- 产后牛子宫感染与皮质醇水平升高有关.
- 具有抗炎和抗氧化特性.
- 皮质醇可以加剧牛子宫内膜细胞的炎症.
研究的目的:
- 为了研究对原生牛子宫内膜层细胞的炎症反应的调节作用.
- 为了确定高皮质醇水平是否影响的抗炎作用.
- 阐明 Selenium 作用背后的分子机制.
主要方法:
- 主要的牛子宫内膜层细胞被用脂多糖治疗以诱导炎症.
- 细胞在高皮质醇存在或不存在的情况下先用烯酸盐进行预处理.
- 使用qPCR测量了类似收费受体4 (TLR4) 和促炎因素的基因表达.
- 对NF-κB和MAPK信号通路的激活通过西斑和免疫光检测进行了评估.
- 分析了包括谷氨过氧化酶在内的蛋白的表达.
主要成果:
- 烯酸降低了TLR4和关键的促炎基因 (IL-1β,IL-6,IL-8,TNF-α,COX-2,iNOS) 的调节.
- 抑制了NF-κB的激活和MAPK通路的酸化 (MKK,ERK,p38,JNK).
- 这些抑制作用在高皮质醇存在时更加明显.
- 调节了谷氨过氧化酶1和4,在联合皮质醇和治疗下观察到更高的表达.
结论:
- 在牛子宫内膜细胞中具有抗炎作用,可能通过NF-κB和MAPK通路进行介导.
- 在这种细胞模型中,皮质醇增加了的抗炎作用.
- 可能提供一种治疗策略,以减轻牛的产后子宫炎症.
相关概念视频
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Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
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01:28Inflammation: Introduction
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