菌碳生成过程通过统计优化的碳酸酶介导和的去除
Naira A Awadeen1, Marwa Eltarahony2, Sahar Zaki3
1Microbiology Department, Faculty of Dentistry, Pharos University, Alexandria, Egypt.
Microbial cell factories
|August 27, 2024
概括
海洋真菌有效地修复重金属 (Zn2+) 和 (Cr6+),使用一种新的碳酸生物沉技术. 这种微生物生物矿化过程为废水处理提供了一个环保的解决方案.
科学领域:
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
- 环境化学环境化学
背景情况:
- 由于重金属污染,工业废水对环境和公共卫生构成重大风险.
- 传统的重金属去除方法往往是昂贵和低效的.
- 海洋真菌菌株为有毒污染物的生物修复提供了一个有希望的途径.
研究的目的:
- 为了研究海洋真菌菌株Rhodotorula sp.的疗效. MZ312369用于 (Zn2+) 和 (Cr6+) 的整治.
- 为了优化碳酸酶 (CA) 酶的活性,以增强生物沉.
- 探索碳酸 (CaCO3) 生物沉对于重金属固定化的潜力.
主要方法:
- 应用普莱克特-伯曼和中央复合材料设计,以优化碳酸酶 (CA) 酶活性.
- 在修复过程中监测微生物数量,CA活性,CaCO3沉量,pH值和金属离子度.
- 使用EDX,SEM,XRD和FTIR分析对生物修复沉物的表征.
主要成果:
- 优化的CA活性达到了154U/mL,增加了1.8倍.
- 在168小时内实现了Zn2+ (50ppm) 和Cr6+ (400ppm) 的完全去除.
- 2+的去除百分比为Zn2+的78.99%,Cr6+的85.06%.
- 矿物学分析证实了瓦特里特 (CaCO) 的形成,封装了重金属.
结论:
- 由CA酶介导的微生物生物矿化有效地在瓦特里特基质中固定Zn2+和Cr6+.
- 这种生物沉技术为减轻废水中的重金属毒性提供了一种可持续和环保的方法.
- 这项研究展示了海洋真菌在重金属修复中的新应用.
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