光蛋白染色体的非退化的两光子吸收
1Department of Chemistry, University of Alberta, Edmonton T6G 2G2, Alberta, Canada.
The journal of physical chemistry. A
|August 28, 2024
概括
非退化两光子吸收 (ND-2PA) 提供了增强的生物成像潜力. 这项研究揭示了FP染色体中的ND-2PA截面可以显著改善退化的2PA,特别是在中性变体中.
科学领域:
- 计算化学是一种计算化学.
- 光物理学的光学物理学
- 生物成像是一种生物成像.
背景情况:
- 两光子吸收 (2PA) 对于生物成像至关重要,通过2PA截面量化 (2PA).
- 退化的2PA (D-2PA) 和非退化的2PA (ND-2PA) 在光子频率上有所不同 (ω 1 = ω 2 与 ω 1 ≠ ω 2).
- ND-2PA对灵活,增强的生物成像有前途,但比D-2PA更少被探索.
研究的目的:
- 在光蛋白 (FP) 染色体模型中研究和比较 σ D-2PA 和 σ ND-2PA .
- 在FP染色体中探索ND-2PA的光物理特性.
- 识别ND-2PA与D-2PA相对的特性中的模式.
主要方法:
- 时间依赖密度函数理论 (TD-DFT) 的计算.
- 二级近似合集群与识别分辨率 (RI-CC2) 计算.
- 从S 0到S 1状态的激发.
主要成果:
- 在CAM-B3LYP (TD-DFT) 和RI-CC2方法中,对于 σ ND-2PA 的改进,几乎有数量一致.
- 计算的σ ND-2PA值在数量上大于σ D-2PA,与两态模型一致.
- 阴性FP染色体显示高达14%的σND-2PA改善;中性FP染色体达到高达30%的增加.
结论:
- 在FP染色体中,ND-2PA比D-2PA具有显著的增强潜力.
- 具有较大的σD-2PA的FP染色体显示出更大的ND-2PA改进潜力.
- 像TD-DFT这样的计算方法可以可靠地预测ND-2PA增强趋势.
更多相关视频
相关概念视频
01:19Protein Dynamics in Living Cells
2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
01:37Super-resolution Fluorescence Microscopy
6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K
01:25Fluorescence and Phosphorescence: Instrumentation
560
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
560
01:14Photoluminescence: Applications
385
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
385