由于在相对和绝对蛋白质量测量中与碳-13和-15标记的同位素合并而导致的偏差

相关实验视频

Updated: Jun 15, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

11.9K

Brian A Rappold1,2, Andrew N Hoofnagle1,2

  • 1Essential Testing, Collinsville, IL 62234, United States.

Clinical mass spectrometry (Del Mar, Calif.)
|August 28, 2024
PubMed
概括

在质谱学中,稳定,同位素标记的内部标准会导致测量不准确. 与分析物相比,制造标准中的自然同位素分布的改变导致了定量错误.

更多相关视频

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling11:53

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Published on: July 1, 2014

16.2K
Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry06:09

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Published on: July 31, 2011

22.9K

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K
Mass Spectrometry: Isotope Effect01:13

Mass Spectrometry: Isotope Effect

Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the difference between the molecular mass. Furthermore, the intensity of these signals is dependent on the...
2.0K