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Rongan Ye¹, Yu He¹, Wei Ni¹

¹Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.

Frontiers in bioengineering and biotechnology

|August 28, 2024

概括

低水平光疗 (LLLT) 通过减少炎症和促进原蛋白,在模拟的微重力中加速皮肤伤口愈合. 这种疗法激活PI3K/AKT通路,同时抑制CCR2,为与空间相关的皮肤损伤提供了一种新的解决方案.

科学领域:

太空医学太空医学
生物医学工程生物医学工程
皮肤病学皮肤病学

背景情况:

随着任务持续时间和宇航员人数的增加,太空中皮肤受伤的风险增加.
了解微重力对伤口愈合的影响对于宇航员健康至关重要.
体重不足给皮肤修复过程带来了独特的挑战.

研究的目的:

研究低水平光疗 (LLLT) 在模拟微重力 (SMG) 下皮肤愈合的治疗潜力.
阐明LLLT在微重力条件下影响伤口修复的分子机制.
为管理与太空相关的皮肤损伤提供理论基础.

主要方法:

使用后肢卸载 (HU) 鼠标模型来模拟无重量状态,有或没有LLLT处理.
在小鼠皮肤组织上进行了组织学分析 (HE,Masson,免疫组织化学).
在SMG下在旋转培养系统 (RSOC) 中使用HaCaT和NIH3T3细胞系进行了体外研究.

主要成果:

在SMG下的HU小鼠中,LLLT显著减少了皮肤伤口面积,增强了原沉积和细胞增殖标记 (Ki67,CD31).
通过降低促炎标记物 (LY6G,F4/80,CD86,IL-1β,IL-6,TNF-α) 和增加一种抗炎标记物 (CD206),LLLT显示出抗炎作用.
生物信息学分析确定了PI3K/AKT信号通路和CCR2作为关键参与者;LLLT增强了PI3K/AKT酸化并减少了CCR2的表达.

结论:

在SMG下,LLLT通过激活PI3K/AKT通路和抑制CCR2来增强皮肤伤口愈合.
在微重力中,CCR2/PI3K/AKT信号轴对于LLLT介导的伤口修复至关重要.
在太空环境中,LLLT为治疗皮肤损伤提供了一个有前途的治疗策略.

关键词:

CCR2 是一个CCR2 的类型.在PI3K中,PI3K是指PI3K.低水平的激光疗法.在微重力环境中,微重力伤口愈合伤口愈合

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