来自参考人群的关节液中和的度的确定和验证
Carrie J Haglock-Adler1, Valentinas Gruzdys2, Frederick G Strathmann3
1Agilent Technologies, Inc., Santa Clara, CA 95051, USA.
Clinical mass spectrometry (Del Mar, Calif.)
|August 28, 2024
概括
本研究使用一种新的基于尿液的校准方法确定了关节液中 (Cr) 和 (Co) 的参考水平. 这些发现为膜液中金属离子分析提供了关键的基准.
科学领域:
- 临床化学 临床化学
- 分析化学 分析化学
- 生物材料科学 生物材料科学
背景情况:
- 精确量化关节液中 (Cr) 和 (Co) 等金属离子对于诊断与植入物相关的问题至关重要.
- 建立可靠的基准水平对于解释患者结果和确保患者安全至关重要.
研究的目的:
- 在参考人群中确定 (Cr) 和 (Co) 的联合流体参考水平.
- 验证一种使用感应合等离子体质谱法 (ICP-MS) 与尿液矩阵校准进行关节液分析的方法.
- 为了分析目的,评估关节液与尿液矩阵的可比性.
主要方法:
- 诱导合等离子质谱法 (ICP-MS) 用于金属离子量化.
- 对方法的性能进行了严格的评估,对不精确性,准确性,线性,分析测量范围 (AMR),灵敏度和转移进行了严格评估.
- 评估了分析和临床恢复,以确认关节液和尿液校准器之间的矩阵可比性.
主要成果:
- 关节液中Cr和Co的参考水平使用97.5百分位数确定:Cr<20.4微克/升,Co<29.9微克/升.
- 该方法证明了可接受的矩阵可比性,允许使用基于尿液的校准器.
- 这项研究成功地克服了对校准器准备有限的关节液体体积的挑战.
结论:
- 开发的ICP-MS方法与基于尿液的校准提供了一种可靠的方法来确定关节液中的Cr和Co水平.
- 确定的参考水平作为临床解释的重要基准.
- 这种方法提高了在关节液中金属离子分析的可行性,特别是当样本体积有限时.
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