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相关概念视频

Oligosaccharide Assembly01:24

Oligosaccharide Assembly

2.8K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
2.8K
Membrane Carbohydrates01:30

Membrane Carbohydrates

5.3K
The plasma membrane is a dynamic barrier composed of lipids, proteins, and carbohydrates. It is the epicenter of many cellular processes required for cell growth and survival. Carbohydrates have unique structural and chemical properties that help the plasma membrane to carry out its functions effectively.
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
5.3K
Protein Glycosylation01:25

Protein Glycosylation

6.8K
Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
6.8K
What are Carbohydrates?01:44

What are Carbohydrates?

170.3K
Overview
170.3K
Chemistry of Carbohydrates03:25

Chemistry of Carbohydrates

72.1K
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
72.1K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.5K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.5K

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相关实验视频

Updated: Jun 15, 2025

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
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Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Published on: September 6, 2019

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基于AIE的精确三元联装,用于糖的识别和分类.

Yongxin Chang1,2, Juan Shao1, Xinjia Zhao1

  • 1State Key Laboratory of Medical Proteomics, National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, P. R. China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|August 28, 2024
PubMed
概括

这项研究引入了一种新的糖类识别策略,使用离子驱动的聚合诱导排放模块. 该方法通过自我组装和光增强精确识别各种糖类,包括异构体.

关键词:
聚合引发的排放量 聚合引发的排放量传感器阵列 传感器阵列 传感器阵列联合组装 联合组装糖的识别方法

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Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System
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Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System

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Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
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相关实验视频

Last Updated: Jun 15, 2025

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Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

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Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System
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Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System

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Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
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Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

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科学领域:

  • 碳水化合物化学 碳水化合物化学
  • 超分子化学 超分子化学
  • 分析化学 分析化学

背景情况:

  • 糖类在生物过程中起着至关重要的作用.
  • 选择性地识别各种糖结构仍然是一个重大挑战.
  • 传统的受体设计往往是复杂和微妙的.

研究的目的:

  • 开发一种新的,精确的三元联合组装策略,用于糖识别.
  • 克服传统复杂受体设计的局限性.
  • 为了创建一个可适应的传感器,用于糖类的分类和分析.

主要方法:

  • 使用了一种素离子驱动的聚合诱导排放模块 (p-toluidine,N,N'-1-propen-1-yl-3-ylidene化物 - PN-Tol).
  • 采用了涉及PN-Tol和4-mercaptophenylboronic acid (MPBA) 的三元联合组装策略.
  • 研究了糖结合时微观结构的形成和光增强.

主要成果:

  • 实现PN-Tol与MPBA的特定结合,形成高度排序的联合组件.
  • 观察到与各种糖类的异质三元组装行为,形成不同的微观结构 (类似集群的,球形的,棒状的).
  • 使用阵列传感器,证明了显著的光增强和成功分类各种糖类,包括epimers和光学异构体.

结论:

  • 新的三元联合组装策略为糖识别提供了一种精确且可适应的方法.
  • 开发的传感器可以有效地对各种糖类进行分类和分析.
  • 这一策略为糖分析和测序开辟了新的途径.