来自Saccharopolyspora hirsuta的双重功能氧化纳米颗粒的生物灵感合成和表征:探索抗菌和抗癌活动
Essam N Sholkamy1, Mohamed A A Abdelhamid2,3, Hazim O Khalifa4,5
1Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Biomimetics (Basel, Switzerland)
|August 28, 2024
概括
这项研究证明了使用actinomyceteSaccharopolyspora hirsuta.使用氧化纳米颗粒 (ZnO-NPs) 的环保合成. 由此产生的ZnO-NP显示出作为双重作用的抗微生物和抗癌剂的潜力.
科学领域:
- 纳米技术 纳米技术
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 微生物合成为纳米粒子生产提供了一个可持续的途径.
- 诸如Saccharopolyspora hirsuta之类的动态菌株因其在生物合成中的潜力而越来越受认可.
- 氧化纳米粒子 (ZnO-NPs) 具有多种生物医学应用.
研究的目的:
- 探索使用从Tapinoma simrothi中分离的Saccharopolyspora hirsuta (Ess_amA6) 探索ZnO-NPs的生物合成.
- 描述合成的ZnO-NP,并评估它们的生物医学潜力,包括抗癌和抗菌作用.
主要方法:
- 使用S. hirsuta. 的 ZnO-NP 的生物合成.
- 通过紫外可见光谱,X射线衍射 (XRD) 和传输电子显微镜 (TEM) 进行表征.
- 对Hep-2和MCF-7癌细胞系的细胞毒性活性和对Pseudomonas aeruginosa和Staphylococcus aureus的抗菌活性的评估.
主要成果:
- 紫外线-Vis光谱学证实了372 nm的ZnO-NP形成.
- 在XRD分析中,发现了六边形的乌尔茨晶体结构,晶体大小为37.5nm.
- TEM揭示了聚合的球形和六角形ZNO-NP,直径为44nm.
- 对Hep-2 (IC50 = 73.01μg/mL) 和MCF-7 (IC50 = 112.74μg/mL) 细胞观察到选择性细胞毒性.
- 对P. aeruginosa (12.67毫米抑制) 和S. aureus (14.33毫米抑制) 进行了广泛的抗菌活性,MIC/MBC值在12.5-50μg/mL之间.
结论:
- S. hirsuta有效地调解了ZnO-NPs的合成.
- 生物合成的ZnO-NP具有显著的选择性抗癌和广泛的抗菌特性.
- 这些发现凸显了S. hirsuta衍生的ZnO-NPs作为双重生物医学应用的生物相容剂的潜力.
关键词:
萨克罗波利斯波拉 hirsutata 的情况.塔皮诺马 (Tapinoma simrothi) 是一种有机的植物.抗菌剂是一种抗菌剂.抗癌剂是一种抗癌剂.生物灵感合成的合成方法氧化纳米颗粒的氧化物.更多相关视频
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