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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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相关实验视频

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Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS
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Published on: May 3, 2011

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影像分光度生物传感器用于无标签的多分子相互作用分析.

Cornelia Reuter1,2, Walter Hauswald1, Sindy Burgold-Voigt1,2

  • 1Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), 07745 Jena, Germany.

Biosensors
|August 28, 2024
PubMed
概括

这项研究引入了一种新的成像衍光度生物传感器,用于无标签检测生物分子. 生物传感器具有高灵敏度,可以同时分析各种目标,如病毒DNA和人体血中的蛋白质.

关键词:
衍射生物传感器生物传感器微分光度成像技术的成像没有标签的相互作用分析.核酸检测核酸检测检测有光学生物传感器.检测蛋白质检测蛋白质的检测

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Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
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相关实验视频

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科学领域:

  • 生物医学工程 生物医学工程
  • 分析化学 分析化学
  • 生物物理学的生物物理.

背景情况:

  • 传统的生物传感器通常需要标签来检测,增加了复杂性.
  • 现有的无标签生物传感器可能会损害检测灵敏度.
  • 需要先进的生物传感平台,提供简单性和高性能.

研究的目的:

  • 开发和验证一种新的成像衍光度生物传感器系统.
  • 为了使各种生物分子的无标签检测和同时分析.
  • 评估生物传感器在复杂矩阵中检测临床相关目标的性能.

主要方法:

  • 开发一个衍射生物传感器芯片和光学阅读器组件.
  • 实施一个无标签测定用于分子相互作用分析.
  • 在人体血中直接检测病毒DNA和蛋白质抗原的应用.

主要成果:

  • 生物传感器成功地将整个测试记录在一个单一的,全面的图像中.
  • 达到4.2pg/mm2的检测极限,与其他无标签的光学生物传感器相当.
  • 在人体血中检测病毒DNA和蛋白质抗原的证明适用性.

结论:

  • 新型成像衍光度生物传感器为无标签的生物分子检测提供了一种强大而简单的方法.
  • 这项技术有可能在分子相互作用分析中广泛应用.
  • 该系统通过提供无标签的同时敏感检测来提升生物传感能力.