用Difenoconazole装载的微囊的抗真菌活性,可以对抗Curvularia lunata
Xiaoyu Chang1, Yuyan Wang1, Abbas Zain2
1College of Resources and Environment, Anhui Science and Technology University, Chuzhou 233100, China.
Journal of fungi (Basel, Switzerland)
|August 28, 2024
概括
奇托桑-迪费诺可纳微囊提供受控释放和增强抗真菌活性对抗Curvularia lunata. 这种新的方法减少了农药的使用,并有效控制了叶斑病.
科学领域:
- 农业科学 农业科学
- 材料科学 材料科学 材料科学
- 菌类学 菌类学是指菌类学.
背景情况:
- 由Curvularia lunata引起的叶子斑等真菌病,显著影响作物产量.
- 传统的农药应用面临着挑战,包括环境持久性和效率降低.
- 开发可控释放配方可以提高农药的效率并减少对环境的影响.
研究的目的:
- 为了合成和表征含有二可纳的奇托桑微囊 (CS-DIF).
- 评估CS-DIF微囊对Curvularia lunata的控制释放性质和抗真菌功效.
- 研究CS-DIF微囊对真菌细胞成分和酶活性的影响.
主要方法:
- 合成含有二可纳的奇托桑微囊.
- 物理和化学性质的表征,包括封装和加载率.
- 通过接触角测量评估可控释放行为和表面稳定性.
- 通过殖民地生长,体外/体内免疫接种以及DNA/蛋白质泄漏的分析来评估抗真菌活性.
- 测量抗氧化酶活性 (SOD,POD,CAT,PPO) 和活性氧物种 (ROS) 水平.
- 在经过处理的真菌中量化可溶糖和蛋白质含量.
主要成果:
- 在CS-DIF微囊中,封装率 (85.58%) 和装载率 (61.98%) 高.
- 观察到显著的控制释放性能,在168小时内仅有33.6%的累积释放.
- 证实了对Curvularia lunata的显著抗真菌活性,显着抑制了生长和破坏了细胞完整性.
- 用CS-DIF微囊治疗导致真菌酶活性发生显著变化,并增加ROS水平.
- 减少可溶糖和蛋白质含量表明真菌细胞严重受损.
结论:
- CS-DIF微囊提供了一种有效的策略,用于控制difenoconazole的输送.
- 微囊对Curvularia lunata具有强烈的抗真菌特性,为叶子斑病管理提供了潜在的解决方案.
- 这种配方提高了农药的效率,减少了所需的数量,为可持续农业和植物保护战略做出了贡献.
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