血激活水对细胞酶产生菌株Aspergillus niger A32的影响
Zhiqing Song1,2, Yingwei Jiang2,3, Chan Chen2,3
1College of Electric Power, Inner Mongolia University of Technology, Hohhot 010080, China.
Journal of fungi (Basel, Switzerland)
|August 28, 2024
概括
血激活水 (PAW) 有效地突变了黑色阿斯伯菌,产生了一种高细胞酶生成菌株. 这种新的方法利用活性氧和物种 (RONS) 来进行微生物繁殖.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 血科学是一门科学课.
背景情况:
- 血激活水 (PAW) 是使用血系统生成的,含有反应性氧和物种 (RONS).
- 研究PAW对微生物菌株的影响对于开发新的生物技术应用至关重要.
- 黑色是一种具有工业意义的真菌,特别是在酶生产中.
研究的目的:
- 调查PAW对黑色菌的作用和机制.
- 使用PAW治疗选和描述A. niger的一种高性能突变菌株.
- 为了比较PAW与含有类似RONS度的化学活性水 (CAW) 的效果.
主要方法:
- 用针阵列板介电屏障放电等离子系统制备PAW.
- Aspergillus niger A32用PAW和CAW进行治疗,随后进行SEM和TEM分析.
- 突变菌株通过序列通道进行选,并测量了酶活性.
- 对所选突变菌株进行了基因组分析 (SNPs和InDels).
主要成果:
- 与CAW相比,PAW的pH值显著降低,ORP和导电性更高.
- 与CAW不同的是,PAW治疗对A. niger造成了显著的形态损伤.
- 获得了一种稳定的突变菌株,A-WW5,细胞质酶,细胞质核核酶和β-葡萄糖酶活性显著增强 (分别高出33%,38%和2.1%).
- 与原始菌株相比,突变菌株A-WW5具有116个SNP和61个InDels.
结论:
- PAW对A. niger的影响涉及长期和短暂的RONS.
- PAW是一种强大的微生物育种突变原体,能够增强工业酶生产.
- 选的突变菌株A-WW5显示出改善细胞酶生产的显著潜力.
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