凝和鱼原蛋白对酸基凝特性的影响:一项比较研究
Adrianna Wierzbicka1, Mateusz Bartniak1, Joanna Waśko2
1Institute of Materials Science and Engineering, Lodz University of Technology, 90-537 Lodz, Poland.
Gels (Basel, Switzerland)
|August 28, 2024
概括
在酸水凝中用鱼类原蛋白取代凝,可以改善组织工程的机械性能和稳定性. 这一创新提供了更好的生物相容性和3D生物打印应用的潜力.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 组织工程是组织工程.
背景情况:
- 甲基酸盐水凝具有可调节的特性,但缺乏生物活性.
- 添加凝可以提高生物活性,但会导致机械性能差,降解速度快.
- 与凝相比,来自鱼类的原蛋白具有更高的生物活性和稳定性,具有额外的安全性和伦理益处.
研究的目的:
- 开发和评估基于酸和鱼类原蛋白的水凝成分.
- 为了比较酸盐-鱼类原蛋白水凝与酸盐-凝水凝的性能.
- 评估这些新型水凝在组织工程中3D打印的潜力.
主要方法:
- 甲基酸盐水凝的配方,含有不同度的鱼类原蛋白.
- 对质,机械和物理化学性质的比较分析.
- 在生理条件下评估胀程度,离子释放动力学和蛋白质释放.
- 基于挤出的3D打印试验.
主要成果:
- 用鱼类原取代凝,导致水凝的胀率降低,机械性能提高.
- 酸鱼原蛋白水凝表现出改善的稳定性和交叉链接离子和蛋白质的受控释放动力学.
- 证明了成功的挤出3D打印,表明了制造复杂支架的潜力.
- 鱼类原蛋白较低的粘度为3D生物打印提供了更好的可加工性.
结论:
- 藻酸盐和鱼类原蛋白的水凝组合物比藻酸盐-酸盐对应物具有更高的性能.
- 鱼类原蛋白的优化度可以为特定的组织工程应用量身定制机械,质和降解配置文件.
- 这些新型生物材料在模仿软组织特性和推进3D生物打印技术方面具有重大前景.
相关概念视频
02:35Acid and Bases: Ka, pKa, and Relative Strengths
This lesson delves into a critical aspect of the relative strengths of acids and bases. The strength of an acid is evaluated by the acid dissociation into its conjugate base and a hydronium ion in water. The complete dissociation of a strong acid is confirmed with a very high concentration of hydronium ions. As a result, an incomplete dissociation process affirms a weak acid. Therefore, the equilibrium is in the forward direction for strong acids and backward for weak acids in these reactions.
02:34Molecular Structure and Acidity
An acid can be deprotonated to form a conjugate base or an anion. If the produced anion is more stable, then the acid is stronger. On the contrary, if the anion is unstable, then the acid is weaker. Hence, to determine the acidity of the compound, the stability of its conjugate base is studied using various factors.
The size effect explains the change in atomic size on acidity. When comparing the acids formed from elements that belong to the same column in the periodic table, their atomic sizes...
The size effect explains the change in atomic size on acidity. When comparing the acids formed from elements that belong to the same column in the periodic table, their atomic sizes...
01:25Acidity and Basicity of Carboxylic Acid Derivatives
Carboxylic acids are the strongest among organic acids, as they readily lose the hydroxyl proton to form a resonance-stabilized carboxylate ion. In comparison, the acid derivatives lack acidic hydrogens directly attached to a functional group. In these compounds, the acidic nature arises from their ability to lose α hydrogens, making them weakly acidic.
The relative acidic strength of the derivatives can be explained based on the extent of resonance stabilization of the conjugate base. The...
The relative acidic strength of the derivatives can be explained based on the extent of resonance stabilization of the conjugate base. The...
01:28What are Proteins?
Proteins are polymers of amino acids linked together by peptide bonds. Proteins and polypeptides are interchangeably used to refer to long chains of amino acids. However, polypeptides have a molecular weight of fewer than 10,000 daltons, while proteins have greater molecular weight. Polypeptides with less than 20 amino acids are called oligopeptides or simply peptides. Interactions among the constituent amino acid side chains of proteins help them fold into a stable 3-dimensional structure...
01:31Substituent Effects on Acidity of Carboxylic Acids
The acidity of carboxylic acids is influenced by the nature of the substituents bounded to the functional group. The acid strength is determined by the stability of the carboxylate anion—the conjugate base formed by dissociating the corresponding carboxylic acid.
01:20Titration of a Weak Base with a Strong Acid
The titration curve of a weak base like ammonia with a strong acid like hydrochloric acid is the mirror image of the titration curve of a weak acid with a strong base.
Using the ICE table and substituting the Kb value, we calculate the initial pH of 50 mL of 0.1 M ammonia to be 11.11. Addition of 25 mL of 0.1 M hydrochloric acid to this solution of ammonia results in a buffer with an equal concentration of ammonia and ammonium ions. The pH of this buffer can be calculated by substituting these...
Using the ICE table and substituting the Kb value, we calculate the initial pH of 50 mL of 0.1 M ammonia to be 11.11. Addition of 25 mL of 0.1 M hydrochloric acid to this solution of ammonia results in a buffer with an equal concentration of ammonia and ammonium ions. The pH of this buffer can be calculated by substituting these...