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衰老介质干细胞的调控作用:内皮细胞反应反应

Andrey Ratushnyy¹, Mariia Ezdakova¹, Diana Matveeva¹

¹Institute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoye Shosse, 76a, 123007 Moscow, Russia.

|August 28, 2024

概括

衰老的介质干细胞 (MSCs) 对内皮细胞 (EC) 的增殖和迁移产生负面影响. 然而,它们的分泌体也可能促进再生,这表明它们在衰老和疾病中的复杂作用.

科学领域:

细胞生物学细胞生物学
衰老研究研究衰老研究
干细胞生物学干细胞生物学

背景情况:

衰老与细胞衰老有关,改变细胞通信并导致组织损伤.
衰老细胞随着年龄的增长而积累,并导致与年龄相关的疾病.
了解衰老细胞与其他细胞类型之间的相互作用至关重要.

研究的目的:

研究衰老介质干细胞 (MSCs) 对内皮细胞 (ECs) 的影响.
为了确定直接的MSC-EC相互作用和MSC秘密对EC功能的影响.
探索衰老在细胞过程中的双重作用.

主要方法:

在MSC中使用mitomycin C (MmC) 诱导衰老.
同时培养了老龄化的MSC与EC.
用老化的MSCs的分泌物对待了EC.
评估了EC增殖,迁移,溶酶体活性,基因表达 (P21,IL6,IL8,ITGA1,ITGB1,ICAM-1),以及生长因子水平 (PDGF-AA).

主要成果:

直接的MSC-EC相互作用减少了EC的增殖和迁移,增加了 lysosomal 活动,并调节了P21,IL6,IL8,ITGA1和ITGB1.
秘密治疗显示出较轻的效果:降低了EC增殖和增加了ICAM-1表达.
衰老的MSC分泌体保持了高水平的衰老相关因素和升高的PDGF-AA,这表明潜在的促再生效应.

结论:

衰老的MSC具有有害的 (减少EC功能) 和潜在的有益的 (促再生因素) 影响.
这些发现突显了衰老在体外细胞行为的复杂影响.
这项研究提供了对衰老和与年龄有关的病理背后的机制的见解.

关键词:

ASC52teloelo的使用情况在 SASP 找 SASP 脑内皮细胞 (ECs) 是一种内皮细胞.中介细胞干细胞 (MSCs)线粒素C (MmC) 的使用.衰老是一种老化.

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