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在自然污染的玉米中,X射线辐射降低了Aspergillus flavus的活力,但并没有降低Aflatoxin B1

Hannah Glesener^1,2, Darya Abdollahzadeh¹, Christopher Muse¹

¹Biodesign Center for Health Through Microbiomes, Arizona State University, Tempe, AZ 85281, USA.

|August 28, 2024

概括

X射线照射有效地减少了玉米中可行的Aspergillus flavus子,玉米是一种常见的粮食作物污染物. 这种方法可以杀菌真菌污染,而不会改变亚毒素B1 (AFB1) 水平,这对于安全的真菌毒素研究至关重要.

科学领域:

食品安全食品安全
菌类学菌类学是指菌类学.
辐射灭菌的辐射灭菌方法

背景情况:

在全球范围内,粮食作物面临着由Aspergillus flavus的污染,这种真菌产生致癌性亚毒素B1 (AFB1).
对AFB1进行准确的实验室研究需要方法来管理真菌污染而不改变毒素水平.

研究的目的:

在实验室环境中评估X射线照射作为AFB1污染的玉米样本的灭菌技术.
为了确定不同X射线剂量对可活性Aspergillus flavus和AFB1度的影响.

主要方法:

自然被AFB1污染的玉米被碎,并以0.0到3.0kGy的剂量进行X射线照射.
使用稀释涂层 (PDA,MDRB) 评估了Aspergillus flavus的活力,并通过qPCR量化基因存在.
使用高性能液体染色学 (HPLC) 和酶相关免疫吸收试验 (ELISA) 测量AFB1度.

主要成果:

增加X射线剂量显著降低了Aspergillus flavus的活力 (PDA和MDRB的p < 0.001;qPCR的p = 0.026).
在玉米样本中,X射线照射并没有显著改变AFB1度 (p=0.153对于HPLC;p=0.567对于ELISA).

结论:

X射线照射有效地减少了受污染的玉米中可行的Aspergillus flavus子.
这种灭菌方法保持了AFB1度,使得对真菌毒素挑战的研究更安全,更可重复.

关键词:

一个. flavususus.非洲毒素 (aflatoxin) 是一种毒素.玉米玉米玉米玉米玉米粮食作物粮食作物辐射辐射辐射的辐射.实验室安全实验室安全

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