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穿透细胞的增强了由蜘蛛毒衍生的神经毒素的杀活性

Wenxian Wu¹, Abid Ali^2,3, Jinbo Shen⁴

¹Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu Agricultural Science and Technology Center, Chengdu 610000, China.

|August 28, 2024

概括

蜘蛛毒HxTx-Hv1h与细胞透 (CPP) -1838融合后显示出增强的杀活性. 这项研究建立了一个用于生产HxTx-Hv1h/CPP-1838的重组系统,这是一个有前途的生物杀虫剂候选者.

科学领域:

生物技术是生物技术.
昆虫毒理学昆虫毒理学
体工程是什么? 体工程是什么?

背景情况:

HxTx-Hv1h是一种蜘蛛毒的神经毒素,是一种潜在的生物杀虫剂.
细胞透 (CPPs) 增强了细胞膜间的分子运输.
毒素与CPP的融合可能会提高生物杀虫剂的疗效.

研究的目的:

为了评估HxTx-Hv1h与CPP-1838.8合并后的虫杀伤疗效.
开发HxTx-Hv1h/CPP-1838融合的复合表达系统.

主要方法:

在酵母中对HxTx-Hv1h,HxTx-Hv1h/GNA和HxTx-Hv1h/CPP-1838的分泌生产.
对虫Megoura crassicauda的接触毒性的评估.
使用BglBrick组件用于重组生产的表达向量的工程.

主要成果:

纯化的HxTx-Hv1h显示接触毒性 (LC50 = 860.5 μg/mL).
与GNA (LC50 = 683.5μg/mL) 和CPP-1838 (LC50 = 465.2μg/mL) 融合显著增强了杀性功效.
添加表面活性剂进一步提高了HxTx-Hv1h/CPP-1838的疗效 (LC50 = 66.7μg/mL).
实现了HxTx-Hv1h/CPP-1838的高水平重组生产.

结论:

CPP的融合策略有效地增强了HxTx-Hv1h的通过皮肤杀虫活性.
重组生产HxTx-Hv1h/CPP-1838是可行的,并有可能用于生物杀虫剂应用.

关键词:

杀伤性阿菲西德 (aphicide) 是一种杀伤性疾病.穿透细胞的可以穿透细胞.融合蛋白质是融合蛋白质的组成部分.杀虫神经毒素是一种杀虫剂神经毒素.多复制表达式多复制表达式

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