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在狗口腔黑色素瘤中通过转录组分析识别出低氧介导的长非编码RNA片段

Yasunori Hino¹, Mohammad Arif², Md Mahfuzur Rahman³

¹United Graduate School of Veterinary Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-0841, Japan.

Veterinary sciences

|August 28, 2024

概括

低氧影响犬口腔黑色素瘤 (COM) 的进展. 研究人员确定了一个特定的长非编码RNA (lncRNA) 片段,ENSCAFT00000084705.1,在COM组织和细胞系中持续下调,但在血中无法检测到.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

缺氧是瘤进展和转移的关键驱动因素.
狗口腔黑色素瘤 (COM) 的预后不好,已知缺氧介导的微RNAs (miRNAs).
有限的数据存在于其他非编码RNAs (ncRNAs) 在COM中因缺氧失调.

研究的目的:

在犬口腔黑色素瘤中通过缺氧调节的非miRNAncRNAs的识别.
调查这些ncRNAs在初级和转移性COM中的表达.
评估已识别的ncRNAs作为生物标记物的潜力.

主要方法:

主要和转移性COM细胞系和临床组织的下一代测序 (NGS).
定量实时PCR (qPCR) 用于验证和量化.
对血和细胞外囊泡 (EVs) 中的ncRNA表达的分析.

主要成果:

在COM中发现,一些非miRNA的ncRNA物种通过缺氧被上调或下调.
与健康的口腔组织相比,COM组织和细胞系中长 ncRNA 片段 ENSCAFT00000084705.1 持续下调.
这种ncRNA在血和血EV中无法检测,这表明瘤组织的特异性.

结论:

这项研究提供了第一个证据,证明了COM中非miRNAncRNA的缺氧转录失调.
ncRNA ENSCAFT00000084705.1是理解COM进展的一个潜在的分子.
瘤组织特异性表达限制了其作为循环生物标记物的潜力.

关键词:

没有NGS,没有NGS.狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗缺氧缺氧是指缺氧的情况.黑色素瘤是一种黑色素瘤.转移转移转移转移这是一个ncRNARNA.

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