相关实验视频

Updated: Jun 15, 2025

In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle

In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle

Published on: July 8, 2020

维生素C改善卵细胞在体外成熟的过程,并可能改变牛的胚胎质量

Yueqi Wang¹, Aibing Wang¹, Hongmei Liu¹

¹State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China.

Veterinary sciences

|August 28, 2024

概括

在牛卵细胞体外成熟 (IVM) 和体外培养 (IVC) 期间补充维生素C可以改善卵细胞的成熟和胚胎发育. 这种维生素C策略增强了多能性基因表达,并调节了与亡相关的基因,可能改善牛胚胎质量.

科学领域:

生殖生物学生殖生物学
发展生物学发展生物学
生物化学生物化学

背景情况:

高质量的牛卵细胞对于高效的辅助生殖技术至关重要.
维生素C (VC) 是一种重要的营养素,具有已知的抗氧化和发育作用.
它对牛卵细胞成熟和早期胚胎发育的特殊影响需要详细研究.

研究的目的:

研究在体外成熟 (IVM) 和体外培养 (IVC) 期间补充维生素C对牛卵细胞成熟和早期胚胎发育的影响.
评估维生素C对牛胚芽细胞中关键多能性和亡相关基因的表达的影响.

主要方法:

牛卵细胞接受了IVM和随后的体外受精 (IVF).
卵子和胚胎在补充了不同度的维生素C的介质中进行培养.
评估了卵细胞成熟率,胚胎裂变率和胚胎囊率.
使用定量PCR分析了多能基因 (Oct4,Sox2,Cdx2,Nanog) 和亡相关基因 (Bcl-2,Bax) 的mRNA表达.

主要成果:

在IVM介质中补充50μg/mL的VC改善了卵细胞成熟率.
在IVC介质中补充20μg/mL的VC增强了试管婴儿胚胎裂解率和胚芽细胞中的多能性基因表达.
联合50μg/mLVC (IVM) +20μg/mLVC (IVC) 策略显著改善了切割率和多能性基因表达.
维生素C补充剂调节了抗亡 (Bcl-2) 和亲亡 (Bax) 基因的表达,影响了Bcl-2/Bax比率.

结论:

补充牛体IVM介质50μg/ml的维生素C可以促进卵细胞的成熟.
在IVM中使用50μg/mLVC和IVC中使用20μg/mLVC的联合方法可以提高牛胚胎质量.
这种维生素C策略对多能性基因表达产生积极影响,并调节牛肉芽细胞中与亡相关的基因表达.

关键词:

这里是牛群.胚胎胚胎是一个胚胎.在体外成熟的成熟.卵子细胞卵子细胞维生素C (VC) 是一种维生素.

更多相关视频

Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation

Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation

Published on: July 30, 2020

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Published on: July 10, 2021

相关概念视频

Oogenesis

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...

In Vitro Fertilization

In Vitro Fertilization

In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...