相关实验视频
Updated: Jun 15, 2025

13:00
Studying Wnt Signaling During Patterning of Conducting Airways
Published on: October 16, 2016
7.3K
Tmem88限制了外皮Wnt2bb信号传递在门门动脉前体中,以平衡细胞周期进展和细胞命运决定
Mingming Zhang1, Jie Liu1, Aihua Mao1
1State Key Laboratory of Membrane Biology, Institute of Zoology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing, China.
Nature cardiovascular research
|August 28, 2024
概括
Tmem88调节门门动脉 (PAA) 的前身发育. Tmem88的丧失导致了过度的祖先增殖和失败的分化,影响了血管形成.
科学领域:
- 发展生物学 发展生物学
- 血管生物学 血管生物学
- 分子生物学分子生物学
背景情况:
- 门门动脉 (PAA) 的祖先对于形成将心脏连接到背上大动脉的血管至关重要.
- 对于PAA原体的扩散和血管细胞分化的编排仍然不完全理解.
研究的目的:
- 调查Tmem88在调节PAA原体的增殖和分化中的作用.
- 阐明Tmem88控制这些过程的分子机制.
主要方法:
- 使用斑马鱼模型研究tmem88a/b的功能.
- 在PAA原始体中分析了基因表达,细胞周期进展和信号通路 (Wnt,cyclin D1-CDK4/6).
- 研究了Nkx2.5/Smad3.5的酸化情况.
主要成果:
- 斑马鱼tmem88a/b的丧失导致PAA前体的过度扩张和血管细胞分化受损.
- 由于异常的Wnt信号传导,Tmem88缺乏导致PAA原始体中环素D1表达的增加.
- 环素D1-CDK4/6活性通过加速G1/S过渡促进了增殖,并通过化Nkx2.5/Smad3.3抑制了分化,从而促进了增殖.
- 发现Tmem88限制了PAA祖先的外皮Wnt2bb信号,平衡了扩散和分化.
结论:
- Tmem88对于微调PAA原体的增殖和分化至关重要.
- 通过Tmem88-Wnt通路和下游细胞循环机制,原生细胞增殖和血管细胞分化之间保持着微妙的平衡.
- 这项研究揭示了PAA祖先的扩散和分化之间的反向关系,PAA祖先由Tmem88.8.调节.
相关概念视频
Canonical Wnt Signaling Pathway
8.7K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.7K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
Non-Canonical Wnt Signaling Pathways
7.3K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.3K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
Notch Signaling Pathway
4.2K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.2K

