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Updated: Jun 15, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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缓解高温LC-MS中列内人工修改,用于自下而上的蛋白质学和蛋白质生物制药的质量控制

Mykyta R Starovoit1, Siddharth Jadeja1, Taťána Gazárková1

  • 1Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Heyrovského 1203/8, 500 03 Hradec Králové, Czech Republic.

Analytical chemistry
|August 28, 2024
PubMed
概括

高柱体温度可以改善分离,但会导致修饰. 这种新的LC-MS方法使用陷柱来减少工件,提高的识别和生物制药质量控制.

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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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