通过液体染色学-双重质谱法在人类母牙中进行类固醇分析,以长期追溯测量类固醇
Ruolan S Wu1,2, Jordan E Hamden1,3, Melody Salehzadeh1,3
1Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, BC, Canada.
PloS one
|August 28, 2024
概括
主牙现在可以分析类固醇激素,提供一种新的方法来研究生命早期的压力. 这种新的测试测量了17种类固醇,提供了对发育内分泌条件的见解.
科学领域:
- 内分泌学 在内分泌学.
- 发展生物学 发展生物学
- 分析化学 分析化学
背景情况:
- 类固醇激素调节重要的生理过程.
- 目前的类固醇测量矩阵 (血液,唾液,尿液) 缺乏长期回顾能力.
- 原始牙为长期类固醇分析提供了一个新的矩阵,这是由于在发育过程中内源性分子的积累.
研究的目的:
- 开发和验证第一个测量测量类固醇在人类初级牙的测试.
- 为了使早期生活压力和发育内分泌病理的回顾性评估.
- 在母牙中同时量化多个类固醇.
主要方法:
- 开发和验证一种液体染色学-并联光谱学 (LC-MS/MS) 试验.
- 在母牙中同时量化17种类固醇.
- 在男性和女性参与者的母牙中进行类固醇分析.
主要成果:
- 开发的LC-MS/MS试验具有敏感性,特异性,准确性和精确性.
- 在17种向类固醇中,有8种在母牙中可量化,其中脱水氨,皮质醇和孕激素是最丰富的.
- 在可量化的类固醇水平中没有观察到任何显著的性别差异.
- 在雄激素水平和葡萄糖皮质激素水平之间发现了正相关性.
结论:
- 多个类固醇可以使用LC-MS/MS.可靠量化在人类初级牙.
- 这种新的方法为回顾性分析早期生活压力的强大工具.
- 这些发现为诊断发育内分泌病理开辟了新的途径.
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