在急性中毒的情况下,通过电膜提取来确定基化物
Xiangting Wei1, Wenyi Liu1, Zhuangzhuang Dong1
1Department of Forensic Medicine, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, 430030, China.
Talanta
|August 28, 2024
概括
电膜提取 (EME) 有效地从生物样本和草药中分离了阿科尼类化物. 这种新的方法有助于识别中毒病例,为法医科学和临床毒理学提供了强大的工具.
科学领域:
- 分析化学 分析化学
- 法医毒理学 法医毒理学
- 药理学 药理学是指药理学的学科.
背景情况:
- 阿基尼丁类化合物 (AC,Mes-AC,Hyp-AC) 是在生物样本和传统中医药中发现的有毒化合物.
- 准确和灵敏地检测这些化物对于法医调查和临床诊断至关重要.
- 现有的化物提取方法可能是复杂和耗时的.
研究的目的:
- 引入电膜提取 (EME) 作为一种新的技术,用于分离乙烯酸化物.
- 开发和验证EME方法与LC-MS/MS和HPLC-UV相结合,用于在各种矩阵中分析这些化物.
- 评估EME在涉及疑似藻类中毒的现实世界法医案件中的适用性.
主要方法:
- 电子膜提取 (EME) 使用1-乙基-2-酸 (ENB) 作为溶剂用于样品制备.
- 该EME方法与液体染色学-并联质谱学 (LC-MS/MS) 和高性能液体染色学-紫外线检测 (HPLC-UV) 相结合.
- 方法验证包括回收,矩阵效应,线性,精度,LOD和LOQ评估在不同样本类型 (全血,尿液,水性) 中.
主要成果:
- EME证明了从多种矩阵中有效提取极性和高分子量酸类化物.
- 恢复率在72%到103%之间,取决于分析物和样本类型.
- EME-LC-MS/MS从0.002 ng/mL实现了低检测极限 (LOD),而EME-HPLC-UV从0.06 μg/mL获得了LOD,精度高 (91%-111%) 和线性 (R2 ≥0.9926).
结论:
- 电膜提取是一种强大而高效的工具,用于从复杂的生物和草药基质中分离氨酸类化合物.
- 开发的EME-LC-MS/MS和EME-HPLC-UV方法适合在法医和临床环境中进行敏感和准确的量化.
- 在法医科学,中国传统医学的分析和临床毒理学中,EME具有很大的应用潜力,特别是在识别中毒病例方面.
更多相关视频
13:35A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
Published on: March 1, 2018
14.6K
09:36Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques
Published on: March 8, 2024
758
相关概念视频
01:14Prevention of Further Absorption of Poison
801
In cases of acute poisoning, the primary objective is to prevent further absorption of the toxic substance into the body. Immediate interventions using various decontamination techniques targeting the gastrointestinal (GI) tract can achieve this. Decontamination is crucial to prevent poison from entering the systemic circulation, which involves washing affected areas with water and mild soap and removing contaminated clothing. Once external decontamination is done, attention must be turned to...
801
00:56Extraction: Advanced Methods
433
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
433
01:26Enhanced Elimination of Poison
489
Poison can be effectively removed from the gastrointestinal (GI) tract through various decontamination procedures.
Antidotes serve a crucial role in counteracting the effects of poison by inhibiting enzymes responsible for producing harmful drug metabolites. In some cases, these toxic metabolites can be neutralized by endogenous cosubstrates, which are maintained at specific concentrations to prevent interaction with cellular macromolecules and subsequent cell death.
Renal excretion is the...
Antidotes serve a crucial role in counteracting the effects of poison by inhibiting enzymes responsible for producing harmful drug metabolites. In some cases, these toxic metabolites can be neutralized by endogenous cosubstrates, which are maintained at specific concentrations to prevent interaction with cellular macromolecules and subsequent cell death.
Renal excretion is the...
489