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光光谱与温度功能测试在评估细胞内能量代谢的标记物:空间异质性和可重复性测量测量
Ekaterina Ryzhkova1, Tatyana Morgunova1, Elena Potapova2
1Department of Endocrinology No.1, Institute of Clinical Medicine N.V. Sklifosovsky, I.M. Sechenov First Moscow State Medical University, Moscow, Russia.
Journal of biophotonics
|August 28, 2024
概括
使用光光谱学 (FS) 测量细胞能量代谢可能是可变的. 前臂皮肤中的NADH光幅度显示出最佳的可重现性,这表明其在评估皮肤能量代谢时的临床使用潜力.
科学领域:
- 生物医学光学 生物医学光学
- 代谢生理学 代谢生理学
- 皮肤病学 皮肤病学
背景情况:
- 细胞能量代谢体现在NADH和FAD++的光强度上.
- 通过光光谱学 (FS) 可以在体内定量这些辅助因子.
- 生理参数的变化会影响测量可重现性和诊断可靠性.
研究的目的:
- 为了评估NADH和FAD++在人体皮肤中的光强度测量的日间可重现性.
- 评估热挑战对这些测量的影响.
- 确定用于评估皮肤能量代谢的临床应用中最可靠的FS参数.
主要方法:
- 一项涉及51名健康志愿者的前性研究.
- 在体内光光谱 (FS) 测量前臂皮肤中的NADH和FAD ++.
- 在局部冷却 (10°C) 和加热 (35°C) 后,在基线上进行的测量.
主要成果:
- 前臂皮肤中NADH的光幅度 (AF_NADH) 在所有研究的FS参数中显示出最高的日间可重复性.
- 热功能测试与AF_NADH测量一起显示出临床应用的希望.
- NADH和FAD++的光强度反映了皮肤的能量代谢,但可重现性有很大的差异.
结论:
- 在背部前臂皮肤中测量AF_NADH是一种高度可重现的方法.
- 将AF_NADH评估与热功能测试相结合,是对皮肤能量代谢的临床评估的一个有希望的方法.
- 这种方法提供了与代谢功能相关的可靠,非侵入性诊断的潜力.
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