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巨型河 (Macrobrachium rosenbergii) 的染色体级的基因组组合

Yang Zheng¹, Guangyu Guo¹, Yanrong Lv¹

¹School of Marine Sciences, State Key Laboratory for Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-sen University, Zhuhai, 519000, China.

Scientific data

|August 28, 2024

概括

研究人员为巨型河 (Macrobrachium rosenbergii) 开发了一种高质量的基因组,该基因组对于通过分子繁殖和抗病能力来改善水产养殖至关重要. 这种基因组资源有助于应对诸如这种重要甲类物种的生殖质降解等挑战.

科学领域:

水产养殖基因组学水产养殖基因组学
甲动物遗传学甲动物遗传学
分子育种是一种分子育种.

背景情况:

巨型河 (Macrobrachium rosenbergii) 水产养殖面临的挑战包括生殖质降解和病毒性疾病.
基因组数据对于开发分子育种计划以改善这种水产养殖物种至关重要.

研究的目的:

为了构建一个高质量的参考基因组Macrobrachium rosenbergii.
为增强水产养殖实践和分子育种提供基因组资源.

主要方法:

集成的纳米孔,Illumina和高通量染色体构造捕获 (Hi-C) 技术.
基因组组装和注释. 基因组组装和注释.
基准测试通用单拷贝正义学家 (BUSCO) 的分析.

主要成果:

一个3.18Gb的基因组组件,具有很高的连接性 (脚手架N50: 62.73Mb,连接N50: 8.92Mb).
98.6%的序列固定在59个伪染色体上,BUSCO得分为94.5%.
鉴定了17436个蛋白质编码基因和43.77%的重复序列.

结论:

高质量的Macrobrachium rosenbergii基因组是水产养殖的宝贵资源.
这种基因组将促进分子育种努力和对十足动物物种进行比较基因组研究.

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

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