细胞球形粘弹性是变形依赖的
Ruben C Boot1, Anouk van der Net2, Christos Gogou2
1Department of Chemical Engineering, Delft University of Technology, Delft, 2629, HZ, The Netherlands.
Scientific reports
|August 28, 2024
概括
细胞球体显示表面张力,这取决于它们的变形程度. 这挑战了表面张力总是随着生物组织中施加的力而增加的想法.
科学领域:
- 生物物理学的生物物理.
- 细胞生物学 细胞生物学
- 组织工程是组织工程.
背景情况:
- 组织表面张力对于细胞分类和融合至关重要.
- 之前的研究表明,多细胞球体在施加力下积极增强表面张力.
研究的目的:
- 调查力持续时间和球状形状变形性对组织表面张力的作用.
- 确定表面紧张强化是否在不同的施加力中一致.
主要方法:
- 使用高吞吐量微流体微管吸收.
- 测量了NIH3T3和HEK293T细胞球体的粘弹性爬行行为.
- 多种应用压力和监测细胞收缩动态.
主要成果:
- 较大的球形变形与更快的细胞收缩后压力释放相关.
- 不太容易变形的NIH3T3球体 (具有更高的α-平滑肌肉活性) 在较小的变形时显示出更慢的收缩速度.
- 尽管粘度增加了,但HEK293T球体仅在更高的压力和变形下表现出收缩.
结论:
- 球体粘弹性取决于变形.
- 在更大的吸入压力下,表面张力的增强是有问题的.
- 这些发现挑战了现有的组织力学和表面张力动态模型.
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