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源叶通过非编码RNA和黄瓜 (Cucumis sativus L.) 中的替代多基化来调节沉强度

Yudan Wang¹, Huimin Zhang², Zhiping Zhang¹

¹College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou, 225009, China.

BMC plant biology

|August 28, 2024

概括

这项研究揭示了非编码RNA (ncRNA) 和替代多基化 (APA) 如何调节黄瓜水槽-来源关系. 这些发现提供了通过分子机制提高作物产量的见解.

科学领域:

植物分子生物学植物分子生物学
植物生理学作物生理学
基因组学就是基因组学.

背景情况:

农作物产量往往受到水槽容量和源强度的限制.
黄瓜是运输拉芬家族寡糖 (RFOs) 的关键作物.
非编码RNAs (ncRNAs) 和替代多基化 (APA) 对于植物生长至关重要,但它们在RFO携带物种的源调节中的作用尚不清楚.

研究的目的:

研究ncRNAs和APA在调节黄瓜中沉积源关系中的作用.
识别不同表达的基因和参与碳分区的调节网络.
为了探索ncRNAs,APA和基因表达之间的相互作用.

主要方法:

来自结果植物 (FNLs) 和非结果植物 (NFNLs) 的黄瓜叶的全转录组测序.
对mRNAs,长非编码RNAs (lncRNAs) 和microRNAs (miRNAs) 的差异表达分析.
同表达和竞争的内源RNA (ceRNA) 网络的构建.
对差异多基 (APA) 基因的分析.

主要成果:

确定了1101个差异表达 (DE) 的mRNA,79个DE lncRNA和23个DE miRNA.
在与光合作用,能量代谢,激素信号传递和碳水化合物代谢相关的途径中,DE基因被丰富.
构建了涉及ncRNAs和APA在下沉源分配中的潜在调节网络,突出了SPS,GBSS1,ERS1,PNO1和Myb等基因.

结论:

一个全面的监管网络涉及mRNAs,ncRNAs和APA在黄瓜水槽-来源关系被阐明.
这些发现为ncRNA和APA的分子机制提供了有价值的见解,用于调节源碳分区的基因表达.
这项研究为未来的研究提供了基础,旨在通过有针对性的分子干预来提高黄瓜产量.

关键词:

替代性的多基解.在CeRNA网络中,黄瓜 - 黄瓜是一种非编码的RNAs.拉菲诺糖家族的寡糖类沉没 - - 来源监管.

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