阴道和子宫内膜微生物组失生症与不良胚胎移植结果相关
Weijue Su1, Chaochao Gong1, Haoyue Zhong1
1Reproductive Medicine Center, Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
Reproductive biology and endocrinology : RB&E
|August 28, 2024
概括
女生殖道微生物影响胚胎植入成功. 乳酸菌的丰富性与成功的IVF-ET有关,微生物分析可以预测植入失败,有助于理解生殖健康.
科学领域:
- 生殖医学 生殖医学
- 微生物组研究 微生物组研究
- 不孕研究 不孕研究 不孕研究
背景情况:
- 辅助生殖技术 (ART) 对不孕症治疗至关重要,但在体外受精-胚胎移植 (IVF-ET) 后的植入失败仍然是一个重大挑战.
- 女性生殖道微生物群在妇科和产科健康中的作用是公认的,但其对胚胎植入失败的具体影响需要进一步研究.
研究的目的:
- 为了研究不育患者的生殖道失生症.
- 为了确定特定的细菌作为胚胎植入失败的潜在生物标志物.
- 通过代谢物分析阐明致病机制.
主要方法:
- 对239名不育患者和17名健康妇女生殖道中的16S rRNA基因和元基因组数据进行比较分析.
- 利用QIIME2和HUMAnN2进行微生物社区和功能概况.
- 建立了一个使用接收机操作特征 (ROC) 分析的诊断模型.
主要成果:
- 在IVF-ET后的乳杆菌丰度和胚胎植入成功 (IS) 之间观察到显著的正相关性.
- 在胚胎植入失败 (IF) 和健康对照患者之间发现了微生物群体组成和结构的明显差异.
- 一种诊断模型在区分成功植入和多重植入失败 (MIF) 之间表现出高准确性,AUC分别为0.913和0.784.
- 甲基因组分析显示,IF患者的L-lysine合成途径的丰富,伴随着阴道pH值的增加和乳杆菌丰度的降低.
结论:
- 这项研究建立了阴道和子宫内膜液细菌与胚胎植入失败之间的独立关系.
- 这些发现扩大了对女性生殖微生物组在生殖健康和ART结果中的作用的理解.
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