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单细胞转录基因组测序揭示了严重结核病患者周围血液免疫细胞的改变

Li Wang^1,2, Ya He^1,2, Peng Wang^1,2

¹Clinic and Research Center of Tuberculosis, School of Medicine, Shanghai Pulmonary Hospital, Tongji University, Shanghai, China.

European journal of medical research

|August 28, 2024

概括

结核病感染严重改变了周围血液的免疫细胞. 严重的结核病显示CD14+单细胞和独特的中性粒细胞增加,CD8+T细胞通过MHC-I和LCK通路表现出强烈的输入相互作用.

科学领域:

免疫学免疫学免疫学
传染性疾病传染性疾病
血液学血液学血液学

背景情况:

结核病 (TB) 仍然是一个重大的全球卫生挑战,由Mycobacterium tuberculosis (Mtb) 引起.
周围血液免疫细胞子集在调节宿主对Mtb感染的反应中起着至关重要的作用.
了解不同结核病严重程度的免疫细胞动态对于有效管理至关重要.

研究的目的:

在患有不同阶段结核病的个体中全面描述周围血液免疫细胞概况.
为了研究在活跃的结核病感染期间免疫细胞的功能和通信变化.

主要方法:

从健康捐赠者 (HD),轻度结核病 (MI) 和严重结核病 (SE) 组中分离周围血液免疫细胞 (PBIC).
流细胞计和细胞通信分析,以识别和量化免疫细胞子集及其相互作用.
对特定细胞标记物和通路的分析,包括T细胞,B细胞,NK细胞,单细胞,中性细胞,MHC-I和LCK.

主要成果:

严重结核病 (SE) 组显示CD14+单细胞增加和独特的GBP5高RSAD2高中性粒细胞.
轻度结核病 (MI) 和SE组表现出CD4+原始T细胞和CD8+T细胞的减少.
增加激活的CD4+ T细胞,过渡性CD8+ T细胞,类似记忆的NK细胞和特定的B细胞在MI和SE组中都被观察到.
CD8+ T细胞在SE组中表现出最高的输入相互作用强度,主要涉及MHC-I和LCK通路.

结论:

结核病感染,特别是严重的结核病,会导致周围血液免疫细胞的组成和功能发生显著改变.
特定的免疫细胞子集,如CD14+单细胞和GBP5高RSAD2高中性粒细胞,可能作为严重结核病的生物标志物.
通过MHC-I和LCK途径增强的CD8+T细胞相互作用突显了它们在对严重结核病的免疫反应中的关键作用.

关键词:

在 CD8+ T 细胞中.免疫细胞是免疫细胞.这就是ScRNA-seqq.严重的结核病严重的结核病结核病是一种疾病.

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