德尔塔-9-四大麻素通过消除反应性氧物种来阻止骨髓衍生的巨细胞分化
Taylor H Carter1, Chloe E Weyer-Nichols1, Jeffrey I Garcia-Sanchez1
1Department of Pathology Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC 29209, USA.
Antioxidants (Basel, Switzerland)
|August 29, 2024
概括
Δ9-四大麻 (THC) 通过抑制活性氧物种 (ROS) 生产来阻止巨细胞分化. 这种植物性大麻素通过调节NRF2-ARE通路并防止铁诱导的ROS形成,影响免疫细胞功能.
科学领域:
- 免疫学 免疫学 免疫学
- 药理学 药理学是指药理学的学科.
- 细胞生物学 细胞生物学
背景情况:
- 巨细胞是关键的免疫细胞,参与病原体防御.
- 巨细胞群刺激因子 (M-CSF) 驱动巨细胞与骨髓衍生细胞 (BMDCs) 的分化.
- Δ9-四大麻 (THC) 是一种来自大麻的植物性大麻素,具有抗炎性质并影响髓状细胞.
研究的目的:
- 为了研究THC对M-CSF诱导的巨细胞分化的影响.
- 阐明THC对BMDCs的影响背后的分子机制.
主要方法:
- BMDCs与M-CSF和THC进行培养.
- 在接受治疗的细胞上进行了RNA测序 (RNA-seq).
- 基于光的测定测量了可变铁和细胞内活性氧物种 (ROS) 水平.
主要成果:
- THC显著抑制了BMDC分化成巨细胞的发生.
- THC的作用独立于已知的大麻素受体 (CB1,CB2) 和其他受体 (GPR18,GPR55,腺素2A).
- RNA-seq揭示了NRF2-ARE基因表达的增加以及与铁和谷氨代谢相关的途径.
- 用THC治疗的细胞显示铁水平增加,细胞内ROS降低,并抑制了芬顿反应.
结论:
- THC阻断了M-CSF诱导的巨细胞分化.
- THC通过NRF2-ARE通路的诱导和防止费顿反应介导的ROS形成来抑制ROS的产生.
- 这些发现揭示了THC免疫调节作用的新机制.
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