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通过冷电子断层扫描和光显微镜对Rhodobacter sphaeroides储存器官进行定量分析

Daniel Parrell^1,2,3, Joseph Olson¹, Rachelle A Lemke^2,3

¹Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.

|August 29, 2024

概括

细菌储存器官,聚乙酸 (PHB) 和聚酸 (PP),在增长停止时,其大小和数量显著增加. 这项研究量化了它们在Rhodobacter sphaeroides中的积累.

科学领域:

微生物学微生物学
细胞生物学细胞生物学
生物化学生化学

背景情况:

细菌拥有多样化的细胞质器官,用于各种功能.
储存颗粒如聚基酸盐 (PHB) 和聚酸盐 (PP) 在细菌生存中起着至关重要的作用.

研究的目的:

为了调查PHB和PP在Rhodobacter sphaeroides中在增长停止的情况下的积累.
为了将这些储存器官的亚细胞定位和丰富度相关联.

主要方法:

低温电子断层扫描 (cryo-ET) 用于在3D中可视化有机细胞.
断层图像的3D细分和分析.
液体染色学和质谱学 (LCMS) 用于PHB量化.
使用PhaP-mNeonGreen聚变蛋白的光光显微镜.

主要成果:

甲治疗的生长停止导致PHB的积累增加了10到20倍.
化ET和光显微镜证实PHB和PP颗粒的尺寸和丰度增加.
观察并讨论了PHB和PP颗粒的同定位.

结论:

细菌储存器官 (PHB和PP) 是动态的,对生长停止有反应.
了解这些有机体有助于进一步了解细菌应激反应和生物塑料.
进一步的研究可以探索可再生能源和生物塑料中的PHB和PP.

关键词:

罗多巴克特球状体 (Rhodobacter sphaeroides) 是一种细菌.细胞的压力是细胞的压力胺尼科尔 (Chloramphenicol) 是一种冷电子显微镜的使用方法冷电子断层扫描 (Cryo-electron tomography) 是一种电子断层扫描技术.聚基酸盐 (PHB) 是一种多基酸盐.聚酸盐 (PP) 是一种多酸盐.

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